FastQCFastQC Report
Wed 15 Jun 2016
000000000-APNWB_l01n01.33100000058c3e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-APNWB_l01n01.33100000058c3e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16822118
Sequences flagged as poor quality0
Sequence length251
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGACACAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA311719518.530336073020056No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA208648512.403224136223512No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA6403663.8066906913861858No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGCGCGCGTA5950303.537188361180203No Hit
TACGTATGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA5438903.233183835709629No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA4304272.558696829971113No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA4009522.3834810812764484No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA3518012.091300275030766No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2671631.5881650574559043No Hit
AACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA2329521.384795897876831No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2143941.2744768524391519No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA2037051.2109355076453512No Hit
TACGGAGGGTCTAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1882921.1193120866230994No Hit
TACGAGATGTGCGAGCGTTGTTCGGAATCACTGGGCATAAAGGGTGCGTA1759781.0461108405017727No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA1569360.9329146306071565No Hit
TACGTATGTTCCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1482690.88139317534213No Hit
TACGTAAAAGACAAGTGTTATTCATGTTAAGTAGGTTTAAAGGGTACCTA1353460.8045716954309796No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATAACTGGGCGTAAAGCGCGCGTA1253920.7453995983145523No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA1134530.6744275601918854No Hit
TACGTAGGTCCCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1118810.6650827202615033No Hit
TACGGGGGGTCCAAGCGTTATTCGGAATCATTGGGCGTAAAGCGCATGTA1102100.655149369419475No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA1090750.6484022998768645No Hit
TACGGAGGACACAAGCGTTATCCGGATTTATTGGGTTTAACGGGTGCGTA1081990.643194869991995No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTAGGTTTAAAGGGTGCGTA1041860.619339372129003No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA1012570.6019277715208038No Hit
TACGGAGGATGCGAGCGTTATTCGGATTTATTGGGTTTAAAGGGTGCGTA948400.5637815642477362No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGCGCATGTA947390.5631811642267638No Hit
TACGGAGGGTCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA912700.5425595041004944No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA886780.5271512184137574No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA695970.41372317088728067No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA695300.41332488572485343No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA695240.41328921839687494No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAACGGGTGCGTA687230.40852763011173737No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA623860.37085698721171734No Hit
AACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA607080.3608820244870473No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA576810.34288785752186496No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATCACTGGGCGTAAAGCGCGCGTA528350.3140805456245165No Hit
TACGGGGGGTGCAAGCGTTATTCGGAATCATTGGGCGTAAAGAGCGCGTA473280.2813438830948636No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA453880.2698114470484632No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA442440.2630108765138849No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA440910.2621013596504317No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA427500.254129711847224No Hit
TACGTAAGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA414570.24644340266784479No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA383160.22777155647106978No Hit
TACGTAGGGAGCAAGCGTTGTCCGGAATGACTGGGCGTAAAGGGCGCGTA335110.19920797131490814No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGAGCGTA331920.1973116583773815No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATGACTGGGCGTAAAGGGCGCGTA330720.19659831181781032No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA312920.18601700451750489No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA305490.18160020040282682No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA299320.17793241017569844No Hit
AACGTAGGGGGCGAGCGTTGTCCGGAATGACTGGGCGTAAAGGGCGCGTA280950.16701226325959667No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA255820.15207359739124407No Hit
TACGGGGGGTCCAAGCGTTATTCGGAATCATTGGGTGTAAAGCGCATGTA251200.14932721313689512No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA234960.13967325636403216No Hit
TACGGAGGATCCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA234450.13937008407621443No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATGACTGGGCGTAAAGGGCGTGTA231870.13783638897313644No Hit
TACGGAGGACACAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA231650.13770560877054838No Hit
TACGTAGGGAGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGGGTGCGTA227650.13532778690531122No Hit
TACGGAGGATACAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA221860.13188588975538038No Hit
TACGGAGGACACAAGCGTTATCCGGATTTATTGGGTTTAAGGGGTGCGTA220650.13116659864114616No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAACGGGTGCGTA215330.1280040955603807No Hit
TACGGAGGACACAAGCGTTATCCGGATTTATTGGGCTTAAAGGGTGCGTA214310.1273977509847452No Hit
TACGGAGGACACAAGCGTTATCCGGATTTATTGGGTTTAAAGGGCGCGTA209520.12455030930112368No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGGGTGCGTA206530.12277288745685888No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGTGCGTA201580.11983033289862788No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATGATTGGGCGTAAAGGGCGCGTA201090.11953904972013632No Hit
TACGTATGTCCCAAGCGTTACCCGGAATCACTGGGTGTAAAGGGTTTGTA186800.11104428110657648No Hit
TACGAAGGGTGCAAGCGTTGTTCGGATTTACTGGGCGTAAAGCGTCTGCA185270.11013476424312325No Hit
TACGTATGTCGCAAGCGTTATCCGGATTTATTGGGCGTAACGCGCGTCTA183780.10924902559832239No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAACGCGCGCGTA180280.10716843146623986No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGCGCGTA179110.10647291857065798No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCATGTA172990.1028348511168451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCC1228200.0244.601046
GACACAA3642400.0244.559428
AGGATGC3770350.0244.532156
GGACACA3651500.0244.412927
ACACAAG3647700.0244.361949
TACGGAG10933950.0244.31321
ATGCGAG2977050.0244.288129
GGAGGAT5073000.0244.145194
GATCCGA399850.0244.111548
TACGTAT897450.0243.962621
ACGTATG898150.0243.936142
AGGACAC3660350.0243.90236
TATGTCG648000.0243.865745
GGATCCG400750.0243.807857
ATCCGAG401050.0243.44229
AACGTAG390950.0243.401981
TAGGTCC145400.0243.3155
GGATGCG2998600.0243.120777
GTCCAAG313550.0243.085639
GTCCCAA158500.0242.835958
Produced by FastQC (version 0.11.2)