FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_vtc5b3.3520000005a37f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_vtc5b3.3520000005a37f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124837
Sequences flagged as poor quality0
Sequence length251
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA4730.37889407787755236No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4470.35806691926271855No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTTGCTTT3460.2771614184897106No Hit
CTTCCTTCTTAATCCGTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG3350.26834992830651166No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2770.22188934370419028No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2660.21307785352099134No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2390.19144965034404865No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2230.1786329373503048No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT2080.16661726891866996No Hit
GAGCAAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACAATGCT1890.15139742223859914No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA1880.15059637767649015No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT1860.14899428855227217No Hit
GGGTACACCATGGACACAGTCAACAGAACACACCAATATTCAGAGAAGGG1810.1449890657417272No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT1790.14338697661750924No Hit
GGTCCCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTT1740.13938175380696427No Hit
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC1720.1377796646827463No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC1720.1377796646827463No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA1520.1217587734405665No Hit
GGTTTCATATCCTGAGCGTAACTTCTCGCTGATCGTTCTTCCCATCCACA1470.11775355063002155No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT1450.11615146150580358No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1440.11535041694369459No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA1440.11535041694369459No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG1410.11294728325736761No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC1390.11134519413314964No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG1370.10974310500893164No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC1350.10814101588471367No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGGATGATGATGGGCAT1340.10733997132260469No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1340.10733997132260469No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA1320.10573788219838669No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC1290.10333474851205973No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGTA100.0014746301244.99998245
CTTACGG100.0014746301244.99998245
GCAGGGT1800.0170.13899
CAGTACG150.00496169163.333339
CGAAAGC950.0128.947373
TACGGAT302.4022232E-4122.52
ACGGATA302.4022232E-4122.53
GCAGGGG704.3655746E-11122.59
GAGCAAA6250.0113.681
CAAAAGC6050.0107.314054
AGCAGGG3850.0101.818188
CTTAATC852.0372681E-10100.8823558
CAGGGGA759.853466E-998.09
AAGCAGG7600.096.7105267
CCGCTTC903.2014214E-1095.277785
AGCAAAA7700.090.6818162
GCAGGTA2250.087.111119
AGCAGGT3550.086.26768
GCAAAAG8050.082.173913
CGGATAA450.001204301381.666674
Produced by FastQC (version 0.11.2)