FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_vtc2c7.3520000005a356.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_vtc2c7.3520000005a356.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138746
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCTTCTTAATCCGTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG8900.6414599339800787No Hit
GAGCAAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACAATGCT7370.5311864846554135No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5130.3697403889121128No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4590.3308203479739957No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC4360.3142432935003532No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTTGCTTT3880.2796477015553602No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT3390.24433136811151313No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC3250.23424098712755684No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3040.2191054156516224No Hit
GATTTAGAAGGTTCCATTGGGAAAGTCTGTAGGACTCTATTGGCTAAGTC2830.20396984417568792No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA2530.18234759921006732No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT2450.17658166721923513No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA2410.17369870122381906No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2340.16865351073184093No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGGTACA2130.15351793925590648No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2110.15207645625819843No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT2060.14847274876392832No Hit
GTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAATGCATGTGTC1990.1434275582719502No Hit
TTTTTAAACTATTCAACATTAATTGATGGCCATCCGAATTCTTTTGGTCG1880.13549940178455594No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA1870.13477866028570193No Hit
CTCTTCCTTCTTAATCCGTCCAGACTCGAAGTCAATTCTGGCATCAATCC1860.13405791878684792No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA1710.12324679630403759No Hit
GCCATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGATGCA1690.12180531330632956No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1670.12036383030862151No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTTT1660.11964308880976748No Hit
GCAAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACAATGCTTC1640.11820160581205943No Hit
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC1600.11531863981664336No Hit
GAGCGAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACAATGCT1550.11171493232237326No Hit
GGTTTGGCCTGCGGAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGG1530.11027344932466522No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC1520.1095527078258112No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT1470.10594900033154109No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT1470.10594900033154109No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG1460.10522825883268706No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA1440.10378677583497903No Hit
AGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAACCC1420.10234529283727099No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA1410.10162455133841697No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCGT100.0014748066245.000021
TCCCGTA100.0014748066245.000022
GCAGGGT1950.0150.769249
CGAAAGC1400.0122.54
GCAGGGG950.0116.052639
CTTAATC1800.0115.694458
GAGCAAA7500.0101.266661
GCAGGTA3050.0100.4098369
CAGTACG501.4897163E-598.09
CAAAAGC8000.093.406254
TTTAAAC801.5439582E-891.875013
TAAACTA801.5439582E-891.875015
ATTTAGA954.9112714E-1090.263152
ACAAGGT552.391079E-589.090917
GATTTAG1007.385097E-1085.751
AAGCAGG10850.084.677427
AGCAGGG4950.084.141418
AGCAAAA9100.082.115382
AAACTAT903.4962795E-881.666676
AGCAGGT5400.079.398158
Produced by FastQC (version 0.11.2)