FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_vtc2c11.3520000005a362.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_vtc2c11.3520000005a362.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150396
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACAATGCT6630.4408361924519269No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA6120.40692571610947104No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG5470.3637064815553605No Hit
CTTCCTTCTTAATCCGTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG5260.3497433442378787No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTTGCTTT4130.27460836724380966No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC3450.22939439878720178No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC3370.22407510838054207No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3040.20213303545307057No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT3040.20213303545307057No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT3030.2014681241522381No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2930.1948190111439134No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2760.18351551902976143No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2490.16556291390728478No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA2270.15093486528897046No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2130.14162610707731588No Hit
GGGTACACCATGGACACAGTCAACAGAACACACCAATATTCAGAGAAGGG2120.14096119577648342No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT2110.14029628447565096No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1970.1309875262639964No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA1930.1283278810606665No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC1870.12433841325567169No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT1850.12300859065400675No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG1820.12101385675150934No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC1790.11901912284901194No Hit
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC1760.11702438894651453No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC1690.11237000984068725No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT1670.11104018723902233No Hit
GTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAATGCATGTGTC1650.10971036463735737No Hit
GGTTTGGCCTGCGGAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGG1550.1030612516290327No Hit
TTTTTAAACTATTCAACATTAATTGATGGCCATCCGAATTCTTTTGGTCG1510.1004016064257028No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTA100.0014749294245.00002245
CAGTACG251.6079866E-9245.09
ACGTAAT150.0049626953163.33333245
GCTGTCC150.0049626953163.33333245
ACGGTCG150.0049626953163.33333245
GTACTAA251.16272975E-4147.0245
CTTAATC1300.0131.923088
GCAGGGT2150.0131.046519
CGAAAGC1700.0129.705894
GCAGGTA4400.0114.147739
GCAGGGG852.0372681E-10100.8823558
AGCAGGT5800.097.155178
GAGCAAA9000.096.638891
CAGGGGA903.2014214E-1095.277789
CAAAAGC9050.092.0442054
TACGGAT403.7441636E-491.875012
GCAAAAG9150.091.0382463
AAGCAGG11900.087.57
ACGGATA457.45933E-481.666673
ACAAGGT603.682841E-581.6666643
Produced by FastQC (version 0.11.2)