FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_vtc10d1.3520000005a38c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_vtc10d1.3520000005a38c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170097
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA10420.612591638888399No Hit
GCCACTGCTCCATTATCTGGACCTGAAATTCCGATTGTTAGCCAGCCCAT7230.42505158821143235No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG6960.40917829238610914No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT5410.3180538163518463No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT4950.29101042346425865No Hit
GCATCCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTT4890.2874830243919646No Hit
ATGTTATCTCCCTCTTGAGCTTCCTATACAGTTTAACTGCTTTGTCCATG3730.21928664232761305No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG3410.20047384727537818No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT3350.19694644820308413No Hit
GATTTCTTTGGCCCCATGGAATGTTATCTCCCTCTTGAGCTTCCTATACA3140.18460055145005497No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA3010.17695785346008452No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA2940.17284255454240816No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT2880.1693151554701141No Hit
GGCTAAAACCATTCTGTTCTCATGTCTGATTAGTGGATTGGTTGTTGTCA2870.1687272556247318No Hit
CTGTTCACAGGTTGCACATACCAGGCCAAATGCCACTTCAGTGGTCACAG2640.15520555918093792No Hit
GTATTAGGGTCCATGGGCCCAGGGTTTTCCTCCACGTCTCCAGCCTGCTT2600.15285395979940858No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2500.14697496134558516No Hit
GTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTG2450.14403546211867346No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG2320.13639276412870305No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG2260.132865365056409No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT2170.12757426644796793No Hit
GGATCACTTGAACCGTTGCATCTGCACCCCCATTCGTTTCTGATAGGCCT2150.12639846675720326No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA2150.12639846675720326No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA2080.12228316783952685No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAAC2050.12051946830337983No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC2050.12051946830337983No Hit
GTACCTGCTTCTCAGTTCAAGTGTACTTGATTCCATAGTCTCCATATTTT1980.11640416938570346No Hit
GCACATACCAGGCCAAATGCCACTTCAGTGGTCACAGCCCCCATCCTGTT1970.1158162695403211No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA1970.1158162695403211No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1950.11464046984955643No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT1940.11405257000417408No Hit
CCCTAATACAGTCTCTAGTTTCCAAGATATACTGCTGAGGATGTCAAAAA1930.11346467015879175No Hit
GTTGTTGTCACCATTTGCCTATGAGACCGATGCTGGGAGTCAGCAATCTG1830.10758567170496833No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG1810.10640987201420365No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA1800.1058219721688213No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1790.10523407232343898No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1790.10523407232343898No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1770.10405827263267431No Hit
GCATTATATGTCCAAATGTCCAGAAATCCATCATCAACTTTTTTATTTAA1770.10405827263267431No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1760.10347037278729196No Hit
GCTTAATACTCTTAGATCTTCAAATGCGGCAGAATGGCATGCCATCCACA1740.10229457309652729No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1720.10111877340576259No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1000.0220.51
GCAAAAG6050.0172.107443
ATCTAGA150.0049632634163.333331
CATGATA150.0049632634163.333336
AGCGAAA1250.0156.799992
GCAGGGG2350.0151.170219
GCAGGGT2150.0142.441859
AGCAAAA7850.0134.203832
AGCAGGG5350.0125.9345868
CAAAAGC8750.0119.04
AAGCAGG12400.0101.754037
CAAGGGT654.832E-794.230774
GTCAATT758.079278E-781.6666642
AAGGGTG603.684232E-581.6666645
GAGCAAA13100.080.4198461
GCAGGTA4650.076.397859
AAAGCAG16600.074.5331346
ATTCGAC500.001830724973.51
AGCACTC2200.072.386379
AGGGTGT707.909237E-570.06
Produced by FastQC (version 0.11.2)