FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_vbz6a1.3520000005a399.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_vbz6a1.3520000005a399.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123846
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5220.4214912068213749No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4520.3649693974775124No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTTGCTTT4100.33105631187119483No Hit
CTTCCTTCTTAATCCGTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG3370.27211213926973826No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2610.21074560341068746No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2510.20267105921870712No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2490.20105615038031105No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2440.19701887828432085No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT2440.19701887828432085No Hit
GGGTACACCATGGACACAGTCAACAGAACACACCAATATTCAGAGAAGGG2170.17521760896597385No Hit
GAGCAAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACAATGCT2110.17037288245078566No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC1930.155838702905221No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT1860.15018652197083474No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA1810.14614924987484457No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT1740.1404970689404583No Hit
GGTCCCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTT1730.13968961452126027No Hit
GGTTTCATATCCTGAGCGTAACTTCTCGCTGATCGTTCTTCCCATCCACA1680.1356523424252701No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT1660.13403743358687403No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC1660.13403743358687403No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA1660.13403743358687403No Hit
ATCATGTACTATGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC1540.12434798055649758No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA1500.12111816287970543No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC1430.1154659819453192No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG1420.11465852752612114No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC1410.1138510731069231No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA1400.11304361868772507No Hit
GGTTTGGCCTGCGGAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGG1400.11304361868772507No Hit
GGATTCTCGTTAGGTCTGATTAGGCTGTATATTTGGCTATTTTGAAGTAG1380.111428709849329No Hit
CATTAATACTGCCCTGCTCAATGCATCCTGTGCAGCAATGGACGATTTTC1310.10577652891494276No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA1310.10577652891494276No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1260.10173925681895259No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1250.10093180239975454No Hit
GGTCACCTCTAACTGCTTTTATCATGCAATCCTCTTGTGAAAACACCATG1250.10093180239975454No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGG100.0014746158245.00002245
GCAGGGG750.0228.666679
GCAGGGT1450.0168.965529
CGAAAGC1200.0163.333334
TGTCGTA150.0049616415163.333332
CAGTACG251.1622372E-4147.09
GCAGGTA2300.0133.152189
GAGCAAA6000.0118.4166641
AAGCAGG7950.0115.566037
GCGAAAG1750.0111.999993
CAAAAGC6650.0106.84214
AGCAGGG4200.0102.0833368
GCAAAAG7100.0100.070433
AGCAAAA7350.098.333332
AGCGAAA2000.098.02
CTTAATC1051.0913936E-1193.3333368
AGGGTGT407.542773E-491.875016
AGCAGGT4000.091.8758
TCTTAAT1101.6370905E-1189.090917
AAAGCAG12000.076.56256
Produced by FastQC (version 0.11.2)