FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_rdentocariosa.3520000005a547.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_rdentocariosa.3520000005a547.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140782
Sequences flagged as poor quality0
Sequence length251
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACTA1107.3717715E-444.5454565
GTATTAG1404.5741843E-543.751
CCATAAC1506.871745E-540.8333323
ACTATCG1751.7028833E-435.08
TATGTAT1400.002412251435.03
CTAGGTT1500.003382967632.6666684
TACAGAA2302.253252E-531.956522
TATACCG1550.00397218931.6129025
TCTACTC1650.005393257429.696973
CCCTAAC1750.007189227727.9999981
TATAGTT1850.00942783126.4864865
CTATCGG2600.00172624823.5576929
TAAGAAC2700.002149934222.6851843
TTAAGAA3403.2146825E-421.6176472
ATACCGA2900.003255297221.120696
GTATTCC3503.9100272E-421.01
AGCGCGC3756.226126E-419.68
CAGGGCG4050.001044581118.1481489
CAATACC3500.00964943817.54
GCGACCA2000.0034887519.8100-104
Produced by FastQC (version 0.11.2)