FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_qiaamp_kit2.3520000005a5e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_qiaamp_kit2.3520000005a5e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287
Sequences flagged as poor quality0
Sequence length251
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTCAGCATCGACGAGTTTTTGTCAAGCGAGTTTACTGTCCTTTGCTG20.1554001554001554No Hit
TCGTTCCAACGCGGGTACTGCTGAACGTCTTCCTCGCCGTCAAACCCAGG20.1554001554001554No Hit
AAGATGTAGTCGGTGAAATACATGAACTGGATATTCCAAATCCTCTATCA20.1554001554001554No Hit
CTTCCTGACCGCCCCGATTGACGATCCCGACGCCGAGCACCTGCGATTCA20.1554001554001554No Hit
GATGACGACGTTGCGGAACCCGTTCTCGCTCACGCGGACGATGGGGACGT20.1554001554001554No Hit
GTTTAAAGAACTTCAGCTTACCGAGCCCTACCTCTCTGACTTTCACAAAA20.1554001554001554No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA5900.09.966103185-189
Produced by FastQC (version 0.11.2)