FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_powersoil_extraction_control.3520000005a5f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_powersoil_extraction_control.3520000005a5f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156
Sequences flagged as poor quality0
Sequence length251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCGATGCACTCGATGCGCGAAATGTGCCACGTTCACGATATGGCAT21.282051282051282No Hit
ATTTTGTGCAGGTTGAAAAGCATTTCAGCCATAATAATTTGCATCGCCAT10.641025641025641No Hit
CATGATCTCAGGGTCCTGGGATCGAGTCCCACATCAGGCTCTCTGCTCAG10.641025641025641No Hit
CTGTCCCCCCAGATGGAAGCAGATGTGCCCTTATCTCCTCTTCATATTCC10.641025641025641No Hit
GAAAGGGGAACCCTCCTACACTGTTGGTGGGAATGCAAGCTGGTGCAGCC10.641025641025641No Hit
CCTGAAACACTCGGTGGTAAGGATGCACACAGTAGTAAATTGGGGCATTG10.641025641025641No Hit
CCAGAGCAGAGTTGAATGATCGGTTGCGGCAGGGCGTGCGCCTCCATCTT10.641025641025641No Hit
AAGACGGTGAATCCCATTTGACTCCGCCGCTGCTTATGGGGATGGATCCC10.641025641025641No Hit
CTCCAGATTTTGGGTAGTCATCCAGGCTTTGGTTCCCATCTGTGTCTCTG10.641025641025641No Hit
AAACTTCACTATTATCCTCCCACCGCACTCACTGACAATTCCTTTTTCAT10.641025641025641No Hit
CTCCAGTCCAATTGAAGCTTTCATTGTTAAACTCCAGTGTGCCGGATGAG10.641025641025641No Hit
GGTGTAGCGAACAGCTTGAAAGAAGGAGAGAAAAAACCGACCTACAACTT10.641025641025641No Hit
TGGTATCCTTGCATTAATTTTATCTCCCGGTCTTCTAGCGAAAGGTACAC10.641025641025641No Hit
CATTTCTGGCATTCCCTGACAACCGCTGGACGAGCGGATGCGGGGTCAGC10.641025641025641No Hit
AGCCATCACCTAGTGACCACCTACTACATTAGGCACTGGGGTAGGCACTG10.641025641025641No Hit
ATGTCATCATGAATACACCTCAGATCACATCAGTTCCTTGGCAAGTAAGT10.641025641025641No Hit
GGGAGGGCCTGGGTCTTCGTCTCCTTGTGCCATCAGATGCCCGGCTGACG10.641025641025641No Hit
ATGGAAAAAGTCCGTGTTCTGTGGTTGTTAATTTGTTTTGTTCTGTTTTT10.641025641025641No Hit
ACGCCATACCGACCACTTCAACTCCATGCTTGTCCCAAGGGATATTGTAA10.641025641025641No Hit
TTGTATATCAATCTGTTGATAAAAGATACGGAGAGGGGCGCCTGGGTGGC10.641025641025641No Hit
TCCGCCTATTTCTCCAAGGCCGACTGGCATACGCCATACTACTGGGCCCC10.641025641025641No Hit
GCCCGAAAACTGCCTCTGGGCCACTGAGCTGACAAGCAGGAGGGGGACTC10.641025641025641No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGTTTCGGATTAGG10.641025641025641No Hit
ATATGTGGGCTTTCATCCTGCTTGGGGTCCAATCCATTATCTTTGTCGTT10.641025641025641No Hit
GGTGAGCCCCGTGCAGCAGGGTGTTGGCTAGCTTCAGTGAAGTGTTTTAA10.641025641025641No Hit
GCTTGTTTCTTCCTATGATCATCTGCAGCAGGCAGAAGATGGGGTTACGA10.641025641025641No Hit
GGCCTAGCACAGACATGTACTGATGGTTAGGACTCAGCAGGGTGGTACTA10.641025641025641No Hit
CATTAACACTATAGATATAATATTTAAAAAGAGGTCGGGACGAAAGTTTT10.641025641025641No Hit
GGGAGAGGGGGCTCCGCAGCCCCCCGCCGGCCTGTGTCCATGCGCCCCCG10.641025641025641No Hit
CACGTGTTTCGGTCCTTCCTCGGGAGAGACCGACATATTGGAAATCAAGA10.641025641025641No Hit
GGGAGAATGATTACGGTGTCATTGAGCAGGGCGTTGTTATGCTTCGCGAT10.641025641025641No Hit
GGGAGTAGGGGCTGAGAGCTGGGCGTCACTTGCTTTTCAAGATTTCCTCT10.641025641025641No Hit
CAAATCCGGGTTTACCGTAAGCCCGGCGGAGGTCGTTATCAATCGAACCT10.641025641025641No Hit
ATATTGAAGCTTCCTCACAAGAAATTGTCGCTTTAAGAAAGGAAGAAAGT10.641025641025641No Hit
ATTTTAACTTAATTGCTTTATTCCAATTGCTTTTTGGTTGGAACCTACAG10.641025641025641No Hit
GAATTTTCTGCCAGCCTGAGGCATCACCAGTGGGTTGGACTGGCTTGGCC10.641025641025641No Hit
TGTCCAGGGCGGGTCATCCGACAGGGTGAGCCTTGGCTTTCCTGATGTAG10.641025641025641No Hit
TGATTACATACAAAAATTAACTTAGGGGCACCTGGGTGGCTCAGTGGGTT10.641025641025641No Hit
ATGCCGATCTAGACATTCATAATTGGCGGCAGGTGTCTTTGTTGTATGTT10.641025641025641No Hit
GCAGTTTGTCAGACGTTTCTCCTCGTTCTTGGCGATAGAGAAGCTGAACA10.641025641025641No Hit
AGGCAGGGTGAAGCTGCCGGGAAAAATCATGCATTTACTTGAAGGCGAAG10.641025641025641No Hit
CTCCGAATCTACCCCCGCCACCCCGCCACCCCGCCACCCCGCCTAGGACT10.641025641025641No Hit
CTAGAGGGCAATGTCAAACTAGTTAAATAAAGTAAACCTATGTCCTATGA10.641025641025641No Hit
GAACATGAGGAGCATAAGGGTACCGTGCATCGTAAAAAGCTGGTTAAATT10.641025641025641No Hit
GGCTGGAGTGTGTCACCAGACAGGGCATGGGTGAGGTGGTTCCAGGACAC10.641025641025641No Hit
CCTTGCCTCTGGTCTCGGCAGTGCTTACCCAAGCAGGAATGCCCAGGGAG10.641025641025641No Hit
CCATGACCTTAACCCTGACCACAACATAACCCTGTCCCTGACCCAAATGT10.641025641025641No Hit
TAGTACTTGATTGGCACAATGGTAATAGAAGTATTCTAAGTGATAGTCAC10.641025641025641No Hit
CTTATAACCACCAGAATTGGAAAGAGAATATGACCTGTTCCTTAAAACCT10.641025641025641No Hit
GGGGAAACAGAGAATCTCCAACGGACCCCAGACCCCCCACTGAACGGGAA10.641025641025641No Hit
GGGTAATAATAGGTCAGGGCTTGTTACTTTGAACCCTGTGCAGACGCTGA10.641025641025641No Hit
GTATACGTTTCGCTCCTGCTGAGAACAATATTAGTGGGATTGCTGTTACT10.641025641025641No Hit
GAACTGCATAAAGACATGCAAATTACAGTAGAAAAGAACGAGATCAATGT10.641025641025641No Hit
GGTCGGTACGCTGCCGACTTTTGACCAGAGCACACTGATTAAATATTATC10.641025641025641No Hit
AGGCAGTACCTGGAGCTAAGTATATGTGTCCATGGTGTGGGTGAGAAATG10.641025641025641No Hit
GGAGCAGACTAAGTCATGCATGACTGAAACTCTCTGGCTTTTTCTTGCTC10.641025641025641No Hit
GCAGTAAAAGCAGCCTCAGTGAGGGCTGTAGGGGACACGGGGATAGGGAG10.641025641025641No Hit
AGATGCCATCTGTCCATCCCTTTCAGTTGGTGTAAATATACAAAGCTGAG10.641025641025641No Hit
AAAAGTAGCTAAGTAAATGAGACCGCTTGTTTTGTACAACCCGCTAAGAA10.641025641025641No Hit
ATGCTGGCGACTCGATTATTGAGGCGTTCACGCTGGTCACCACGATGGCG10.641025641025641No Hit
GGCAGAGGCAGGGGCGGGACTTCGCGGAAGGAGCGGAGCTGGCTGAGGCC10.641025641025641No Hit
CTATCAAATTTAAGGGCACAGGTAAATGAAAATGCAGTAATACAAAGGGT10.641025641025641No Hit
GTTATATTCCCTGTCCCGACACGGGCAGCCATACTAATGGCAAATGCCTG10.641025641025641No Hit
GAAAACTGAGGTTGGGAAGAGATGAAGACCTAAGGCCATGGTGTGACTGA10.641025641025641No Hit
GGGGGATCCCGTCAGATCTAAGATCTTTTTGGGCCCCCTTTTGTTAAGAA10.641025641025641No Hit
ATTTGGTAGTCTTTGAGCAGGATCTTAGATTTTCATGGAATGAGGGTAAC10.641025641025641No Hit
GTTCTCCGGAAGAAACCGAGGCCCGGCTCAGGGACATCCGCTCACAGATC10.641025641025641No Hit
AATGGGACTTGATCTCATGACCCTGAGATCATGACCTGAGCCAAAATCAA10.641025641025641No Hit
ATAAGCGAAAGTGCCGTGGATGCGAGCATGCCGTAGAGAATAGCGCCGCG10.641025641025641No Hit
AAAGAGGGCTGATCCCTTCTCCTCCCAACTCAGCCTGGCACTGCTCTGGC10.641025641025641No Hit
ATGTATGACATCCAATACATTTGTCTAAGTTCAGTACCATTGCTATCTGC10.641025641025641No Hit
CCATCCAACAGATTGACGAAAAACAATAGGGATCGCGCCAAATCCACAGA10.641025641025641No Hit
CCCTCGGACTCACCGTGATGAATACCGGCACGATCTCTGTTCTGGGCAAG10.641025641025641No Hit
ATGTCATTGAAGCATTGATTAAAGGTCATATCGTCGGGATTGCAAAGTAC10.641025641025641No Hit
AAAAAACCCTTCCCTGTGGGGTTATCTGAATGTGCATTGTATCTTCTGAA10.641025641025641No Hit
GGCCATGGCGGGTCTGATATGCTCTGTGGGGTGGGAGGACTCTTTCAGGT10.641025641025641No Hit
GTTTGAAACAAAGTCCAGGTGGATCAGCATTTTCCAGCAAAGGGTCAGAG10.641025641025641No Hit
GTATATTGCCTAAAGTGAAATAGCTGGGAAGTATCTGCTTCATCAGGAAT10.641025641025641No Hit
GATTATTCGGGGGAATTTGCCTGAGAATTATGCGCAAGATGTTCATACGT10.641025641025641No Hit
CCATTATGGTGGGGGCCGTCATCGCCATGATGCGCGGGCGTGAAGACATC10.641025641025641No Hit
GGGCATGTTGATTGGCGGCGTGCTGGCCGCCGTGATTGCAGGGAGAGTGA10.641025641025641No Hit
GGTAACTCCTCGACAAAAGATCGTGTGTATGAGACGCGGTAACTTCATCT10.641025641025641No Hit
CCTATTCCCTTCCTTTTGAGCAGGTGGATCAAGTGGTGGATACCGTGGGC10.641025641025641No Hit
TCCGAGCACATTCACAGGCAGCTTCCCCACAAATTCCACTAGCAACCCAG10.641025641025641No Hit
GAATGGACTTTCGACGCTGGGCTTATGGTTCGTGAACGTCTGTACCGCCG10.641025641025641No Hit
CGACTACGCCTTCCGTTACCGTCTTCGGCAGCTTGAGGCCCTCCTTCTCC10.641025641025641No Hit
AAATAAGCTGAAATGAGAAAGTTCTTATCTCCTGTTCCACTTCAAACAGT10.641025641025641No Hit
CAGCGAGGCGTTCGCGCCCGAAAGCGCGGCGATCAAAATCACGAAGTTGG10.641025641025641No Hit
GTCTAATACAGGTTAAGCAGACAAAAATGAATAGGTCTTTAGAAGGTCGA10.641025641025641No Hit
GCAAAGCCCGGGGCGTGTGGCTATACTATGAGAGGATAAGATGCCGCAAA10.641025641025641No Hit
GTTTCATCAAAGCACACATTCACATCCCGCAGAGAGGCTGAAGGCGGTGA10.641025641025641No Hit
GGTATCAGCTGGTGGCTGGGCCAGCTCAGGCCCCCTGCAGGAGAGGGTGG10.641025641025641No Hit
TTTATTGAAGGTTTTGCTGAAAAACAAGTACCAGCAAGAGACTTTATTTT10.641025641025641No Hit
CCTTGGATACCGACATTTGGAATATTTCAGCGAGCTGCTTTAATGAAATG10.641025641025641No Hit
CTCTATGGGCGGTCATGGTGCTTTAGTAATTGGGTTGAAAAATACAGAAA10.641025641025641No Hit
CAGTTCCACGATGACGCCGGATACACCGCTTAATGAGTCCGTGATTTGAG10.641025641025641No Hit
CCACCAGGGAAGGAGAGAGGGGAAGAGAAGAGGGAGAGAGGGAGTAAAGG10.641025641025641No Hit
TTCCGGAACCGAAGCAGCAGTTTGTGTACGGCCTCGATCAAACCGTCTTT10.641025641025641No Hit
ATCCCAGCTCAGTCACACTGAAGGTAGACCTTTTCGCTCTCTCCCTCCAT10.641025641025641No Hit
GAGCCACCCAGGTGCCCTAGCCTCAGTTTAATAATAGGTACATACTTCAT10.641025641025641No Hit
GCAGTATAGACATTTGAAAAGATTTTTTAATTTACTTGACAGAGAAAGAG10.641025641025641No Hit
GTCTAAGGAGATCATGAGCATTCATGATCTCCTTTTTGTTGTTTTCTGAT10.641025641025641No Hit
CCAGGGAGACAGGAGTGACTGACTGCTTGTCTCTGAGGGCACACTAGGAG10.641025641025641No Hit
ATACCGTACTAACGAGGTGTCGGTTAAACAGATGAGTGATGTCTCTTTTT10.641025641025641No Hit
GCGAGGAACTCCTCGATCTGCTTCTGCACTTCTGCCTTAACAATATTCAG10.641025641025641No Hit
GTGGGTTAAGCCGCTGCCTTCGGCTCAGGTCATGATCTCAGGGTCCTGGG10.641025641025641No Hit
CCATAAATTTCAAATGTGGCAGCATCAAACGCTACAGTTCCCGATAATAA10.641025641025641No Hit
GTGATACACATAGAAGTCACTGTATCATGGCAGATAACAGCCAGAACCAG10.641025641025641No Hit
GCCATTACCTGTTGAATTATGACTCCTCTGCCTGTAATCTCTGTTTTAGA10.641025641025641No Hit
GTCCCCCTCTGTTCACTCTTCACCAGTGGCTTGGGCAGGATTTGGTGTTG10.641025641025641No Hit
GATTCTCGCAGTACTCCTTGCCATGGTCTTGCACACAATGCAAGCATGCT10.641025641025641No Hit
GGCTTCGGCAGAAGAGGCTGTGCAAGGCGCGATGGATATACTGGCCGAGA10.641025641025641No Hit
ATTATACTCCTGAAAATGACTGAAAAAAGACAAAATAAGCAGAAGAAACA10.641025641025641No Hit
TCTAGATTCCTATCATCCTGGCATTGGCTTGGATTCCTCCTCCTGGTTAT10.641025641025641No Hit
CTTATATATAATTTCAGTATAATCTCTTTTCATTTTTTGTTGTTCAGTAG10.641025641025641No Hit
CTATTAAACTCGATGAACCGGGATGTGGCGGAGATATTCGATACGTACGT10.641025641025641No Hit
CTAGAATTGCTGTTCTTCTTCTCTTTGATCTGCCAATGGACTGTCTCTTA10.641025641025641No Hit
AGGTTACACAGGGCAGAGGAGAGGGCCAGATGGAGGGAGGGCAATGCCCT10.641025641025641No Hit
CTTCTGGGGGGGTCCTCCACGGGGGCCCTACAGCTGCACGGACGTGTCTA10.641025641025641No Hit
CAGCACTAGAGAGGAAAAGCAATCCATAATCTCAACAATTTCAACTCTAT10.641025641025641No Hit
GGAATACAAGCAGGAGTGGATAGCTTCTATAGGACCTGCAAGTTAGTGGG10.641025641025641No Hit
CACCAGGGCAGGGCGGCGTCTCCCGACCGGGTTTCCTACTTCCAGCTGTC10.641025641025641No Hit
GAGTAAGGTAGAGAAAACTGAGACACTTATTCCACTGCCTGGTCTGAGGA10.641025641025641No Hit
ATTATATTCTTTATCTTCACCTTCAAGTACATAAACAGGCGCTTCGTCAG10.641025641025641No Hit
ACGTCCAGGAGCACGCCCTCGATCTCATCCAGCGTCTCTTTTTCGAGAAT10.641025641025641No Hit
GATGAGATGTTTACCAGGCACACATTGTTTCGTCAGTTTGCCATGCTTGC10.641025641025641No Hit
CTATAGATAGGTATTGAATATCAGCAATCTGCTTGCGCATCGCATCAAAT10.641025641025641No Hit
AAATCCGGAAGATCATTGATCGTCAATGCCTTGCCCTGATCATCCCCGAT10.641025641025641No Hit
GACACTGGCACGGAGACCACCAGGGCGCCCACTGCCGTGCGGGCAGGGCA10.641025641025641No Hit
ATTGAAATGAGTAAGATACCAATGAATGGTGATGATGAAGCTCAGAAACA10.641025641025641No Hit
GTGCACATTTGGATGTAGAATCTCCCAATTCCATCAATCATCTTCCCGAC10.641025641025641No Hit
CCATTCCTTGGCGTGATGCCTTACAAGCCGGACCGCACGCTGGGCATCTC10.641025641025641No Hit
GGAGCATCCCTGGGGATTCTGCTTATGGTTTAATACATTCTGTCCCTCCA10.641025641025641No Hit
GTTAGAATAAATGAAAGCCTGATACCCACTGAATAGAATAATGCACCAAG10.641025641025641No Hit
CAAAGGAGTCACCGATCCGGATCAGCAGAAACAGCAGTTTATCAGCCTGC10.641025641025641No Hit
TTTGAGGTAGAACACGCAAAAGAGAGAGCACAAGCTGGGGTACAGAGGGA10.641025641025641No Hit
ACAGCGGGTCGTCTACGGTGCGCATCGTGAGTATGCCGCTTTCAGACAGC10.641025641025641No Hit
CCACTTTTCTCTGGCTTCTAGGGCTCAGCTTGCTCTGCCTACCAGGTGGT10.641025641025641No Hit
TATCCGAATCATCCCCGTAGTTATTGTTCCAGTCGAGGCACGCAGCCGGC10.641025641025641No Hit
GAGCTGAAAAGTACAAGTCACCGGGGGTAAGGGGTGGCAGGATGGGGGCC10.641025641025641No Hit
CAGGAAATGTGCCCCATAGTTGAAGGCATCTTTCTTGGCACTTCCCTTGG10.641025641025641No Hit
GAGGCACCCAGGTGCCCCTAGGAATGTAAGGGCTTTAAATGACACATTGG10.641025641025641No Hit
GCTCCGGGCCCGCGTACCGATCCATGAAATAATGCGCAAAAATCCCTGCC10.641025641025641No Hit
GGGAATAGGGCCGAGGGCAGATAGACACTGGGCAGGCTCCAAAGCCAGGG10.641025641025641No Hit
GGGAAAACTGACCACTCACCCCACTCCTCTTCCCTTAATTTTAGAATAAG10.641025641025641No Hit
ACAGACATAGTATGGATATTAAGTCTGTTTGATGTTTCCAGTTAGTCCCT10.641025641025641No Hit
ATATGTATAGATTGTGCCGTACGATTAGGGTCTATACGCTGATATAATTC10.641025641025641No Hit
GCTGCTTCCAAAACTCAGTGTTATCATCTACCCGGTCACCTCGGGTCCTG10.641025641025641No Hit
GGCGAAATATGTTTTGTAAACAAACAAGACTCATGGGAAGCTTTTTTGTA10.641025641025641No Hit
CTCGCTTGTCACGTATCGGCCCAATCCCCGCCGCGTCTCCACAATACCCT10.641025641025641No Hit
GTCTGTGGTCTCAGCTCCGTTCACTTTGGCTCCCGGAGCCGGGGTGAGCT10.641025641025641No Hit
GCTCATCCGTGGACAAACCGAGTTTGTCCGCTTCCTGTACCATAGGCACC10.641025641025641No Hit
GTCTCTCAATATTGTCCTCTGGTGAAATGAAAGGGGGAATTATAATTCAG10.641025641025641No Hit
GAGAAAAACCAAGGAAGTAATAATGCCAACTATGATAATATCTGCTTGGG10.641025641025641No Hit
ATGGAGACCTTGTAATGGGGCAGTGGCACACAGGCCCTGAAGGATTCTCC10.641025641025641No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA650.00218840418.846155190-194