FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_pacnes.3520000005a3b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_pacnes.3520000005a3b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107708
Sequences flagged as poor quality0
Sequence length251
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAG251.1618204E-4147.01
CCCTAGA450.001203850381.666672
CTAGTAC500.001828895173.53
TCCTAAG550.002668984766.8181842
CTAGACT650.005172760756.538464
TAGCGTC650.005172760756.538469
CTTAGGG650.005172760756.53846245
CTGTTCT700.006935028452.59
GAACACG700.006935028452.5245
TTGACTG750.009109369549.0000047
TAAGTAG1159.1658044E-442.6086965
AGAGACA1300.001674894137.6923076
TAGACTA1350.002015901736.29635
TACTGCT1500.003379462532.6666687
TATCCTA1500.003379462532.6666685
TATACTG1650.00538769729.696975
TATAACC1650.00538769729.696972
CATAGAA1800.00824023227.2222232
GTATATA2359.562217E-426.0638281
CAATACC2750.002388200522.2727284
Produced by FastQC (version 0.11.2)