FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_ny238plasmidsmix1rep.3520000005a349.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_ny238plasmidsmix1rep.3520000005a349.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92850
Sequences flagged as poor quality0
Sequence length251
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG2340.25201938610662356No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA2280.2455573505654281No Hit
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA2140.23047926763597198No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT1730.18632202477113624No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT1630.17555196553581046No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT1590.17124394184168013No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC1530.16478190630048467No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT1380.14862681744749598No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG1250.1346257404415724No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT1140.12277867528271405No Hit
TTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCC1090.11739364566505117No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC1060.11416262789445342No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC1060.11416262789445342No Hit
ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG1050.11308562197092084No Hit
ATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT1050.11308562197092084No Hit
ACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATA1010.10877759827679052No Hit
TGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATC1010.10877759827679052No Hit
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGA930.10016155088852989No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTCG150.0049596555163.333335
TTAGATA354.4323254E-4105.000015
CTTATTA354.4323254E-4105.000011
TACGCGG407.5367815E-491.87501245
AGATAGC450.001203323781.666677
ATATAGT707.890355E-570.000016
GTTCTAC550.002667822466.8181846
CATATTA801.5279677E-461.2500045
CCATATT801.5279677E-461.2500044
ATCCCAT1006.230046E-661.251
AGGGAGG600.003766156561.257
GATAGCC600.003766156561.258
TATATAT1759.658834E-1055.9999962
TAGGGGA700.00693202752.5000044
GTAGGAC1004.600251E-449.08
GTAATAC750.00910543949.01
GTATATA2509.094947E-1249.01
ATTATTA1055.851372E-446.6666643
CTTATAT1353.680528E-545.3703733
TAGGACA1107.3587557E-444.5454569
Produced by FastQC (version 0.11.2)