FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_ny238cvp.3520000005a3a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_ny238cvp.3520000005a3a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132483
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG6050.4566623642278632No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5430.40986390706732184No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTTGCTTT3990.3011707162428387No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC3720.28079074296324813No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC3240.24455967935508707No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT3040.22946340285168662No Hit
CTTCCTTCTTAATCCGTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG2940.22191526459998642No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2720.20530936044624595No Hit
ACATAATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGTTC2660.2007804774952258No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2370.17889087656529518No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT2340.1766264350897851No Hit
GGTCCCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTT2240.1690782968380849No Hit
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC2200.1660590415374048No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT2040.15398202033468444No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC1980.1494531373836643No Hit
GGGTACACCATGGACACAGTCAACAGAACACACCAATATTCAGAGAAGGG1920.1449242544326442No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC1900.14341462678230413No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT1830.13813093000611398No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT1800.13586648853060393No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA1790.1351116747054339No Hit
GGTTTCATATCCTGAGCGTAACTTCTCGCTGATCGTTCTTCCCATCCACA1790.1351116747054339No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA1780.13435686088026388No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA1770.13360204705509385No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA1760.13284723322992384No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC1640.12378946732788357No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC1590.12001539820203347No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA1530.11548651525101333No Hit
ATTATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC1470.11095763229999321No Hit
CTATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTG1470.11095763229999321No Hit
GGTTTGGCCTGCGGAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGG1460.11020281847482317No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1460.11020281847482317No Hit
GGATTCTCGTTAGGTCTGATTAGGCTGTATATTTGGCTATTTTGAAGTAG1410.10642874934897308No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG1410.10642874934897308No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT1400.10567393552380305No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG1400.10567393552380305No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT1340.10114505257278292No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT100.0014747318244.999982
ACGGATA100.0014747318244.999983
GACGCTC100.0014747318244.99998245
CCCTACA150.0049620313163.33333245
GCAGGGA150.0049620313163.333339
GCAGGGT2400.0142.916679
GCAGGGG652.3646862E-11131.923089
TACATGG404.913909E-6122.499992
GAGCAAA5450.0121.3761441
AGCAAAA6550.0100.992362
TAGGACC1950.0100.512829
AGCAGGG4600.098.532618
AGGGTGT407.5439474E-491.874996
CAAAAGC7350.090.000014
CGAAAGC1500.089.8333363
CGACGAA450.001204465481.66667245
CATGGTA603.681217E-581.6666644
GTACATG1650.081.6666641
GGTAGGA603.681217E-581.6666647
TGGTAGG603.681217E-581.6666646
Produced by FastQC (version 0.11.2)