FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_ltcontamination.3520000005a554.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_ltcontamination.3520000005a554.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147624
Sequences flagged as poor quality0
Sequence length251
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGCG150.0049626036163.333339
GTGACCC600.003770560361.259
CTAGGGG700.00694009852.5245
GTCCTGG1159.176882E-442.6086961
GACGAAA2802.4494202E-630.625245
ACGGCTC1600.00464054630.6249988
TATACGT1800.0082499727.2222235
AATTGGA2359.579096E-426.0638282
GAGAGGG3453.5490873E-421.304348245
ATATAAT3453.5490873E-421.3043481
TTGGAAG2950.003595206420.7627124
GGGAGAC1654.6934067E-513.36363760-64
CCCCCCC3152.1318454E-411.666667245
ACACACA2451.641168E-410.0235-239
CACACAC2000.0034890939.8240-244
TTTGGCG3152.1318454E-48.55555570-74
Produced by FastQC (version 0.11.2)