FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_gv1291.3520000005a1b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_gv1291.3520000005a1b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139642
Sequences flagged as poor quality0
Sequence length251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA38372.7477406510935105No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA24691.7680926941751047No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC16401.1744317612179718No Hit
GAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA15771.12931639478094No Hit
AGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT14751.0562724681686027No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT11230.8041993096632819No Hit
GTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGC10460.7490583062402429No Hit
GCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC10230.7325876169060884No Hit
GGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTT7390.5292104094756592No Hit
AGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT7340.5256298248377995No Hit
ACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCA6970.499133498517638No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC6530.4676243537044729No Hit
GATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGT6140.4396957935291675No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG5680.4067544148608585No Hit
AGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCA5310.3802580885406969No Hit
CCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCAA4700.336574955958809No Hit
ATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAAAG4680.3351427221036651No Hit
CATTTAGGGCATTTTGGATAAAGCGTCTACGCTGCAGTCCTCGCTCACTG4510.3229687343349422No Hit
ATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAAGA4400.31509144813165096No Hit
CTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATG4230.30291746036292805No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG4050.29002735566663324No Hit
TGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAAC3970.2842984202460578No Hit
GTATCAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG3630.259950444708612No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA3610.25851821085346816No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAT3600.2578020939258962No Hit
CTTGAATGGAATGGTAACACAGTTCGAGTCTCTGAAAATATACAGAGATT3440.2463442230847453No Hit
GTATTAATTGATGGCCATCCGAATCCTTTTGGTCGCTGTCTGGCTGTCAG3430.24562810615717331No Hit
TTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTT3410.2441958723020295No Hit
GATTCGCTTGGAGAAGCTGTGATGAGAATGGGAGACCTTCATTACCTCCA3410.2441958723020295No Hit
GACTTGAAGATGTCTTTGCAGGAAAGAACACAGATCTTGAGGCTCTCATG3290.23560246917116628No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA3260.23345411838845048No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGAAAAG3220.23058965067816276No Hit
AATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCA3150.22557683218515917No Hit
AGATACGGACCAGCATTAAGCATCAATGAACTGAGTAACCTTGCAAAAGG3130.22414459833001535No Hit
GATTACTGATCGTCTTTTTTTCAAATGTATTTATCGTCGCTTTAAATACG3070.21984789676458374No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT3040.21769954598186794No Hit
CATTTAGGGCATTTTGGACAAAGCGTCTACGCTGCAGTCCTCGCTCACTG2960.21197061056129246No Hit
ATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTT2930.20982225977857666No Hit
GAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTA2840.20337720743042925No Hit
ATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAA2800.20051273972014153No Hit
GGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTG2800.20051273972014153No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG2730.19549992122713794No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA2650.1897709858065625No Hit
CTAAAGGAATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAGTGAGCGA2640.18905486887899056No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCGCCGGCA2600.18619040116870283No Hit
TAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGC2580.18475816731355896No Hit
CAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGAT2530.18117758267569928No Hit
ATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCATCATGTGGGCCT2520.18046146574812735No Hit
GGCGAGAGCAATTGGGACAGAAATTTGAGGAAATAAGATGGTTAATTGAA2450.17544864725512382No Hit
GCTTGTTGTTGCCGCGAATATCATTGGGATCTTGCACTTGATATTGTGGA2440.17473253032755187No Hit
GTATGGAAAGAGTAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT2440.17473253032755187No Hit
GCTTTGTCCATGTTATTTGGGTCTCCATTCCCATTGAGGGCATTTTGGAC2430.17401641339997995No Hit
AAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATG2400.17186806261726414No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT2380.1704358287621203No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2370.16971971183454834No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT2320.1661391271966887No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC2310.16542301026911674No Hit
ATCCCCTTAGTCAAAGGTGACAGAATTGGTCTTGTCTTTAGCCATTCCAT2310.16542301026911674No Hit
CGTACGTTCTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCG2270.162558542558829No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG2260.1618424256312571No Hit
GAAATAGCTCTCAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCT2240.1604101917761132No Hit
TACAGCCCCCATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCAC2240.1604101917761132No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG2190.15682960713825353No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC2150.1539651394279658No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC2150.1539651394279658No Hit
ATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGG2140.15324902250039388No Hit
TCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCAT2090.1496684378625342No Hit
TGTTTCTACTAATAACCCGAGTAGAAACAAGGGTGTTTTTAATTAATGCA2080.14895232093496227No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT2080.14895232093496227No Hit
ATTATATTCAGTATGGAAAGAGTAAAAGAACTACGGAATCTAATGTCGCA2070.14823620400739032No Hit
GAATCCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATACTAGAGTCCCGT2070.14823620400739032No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA2060.1475200870798184No Hit
GTGTTGCTTTGTTGGGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATT2050.14680397015224647No Hit
GCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCATCATGTG2040.14608785322467452No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2020.14465561936953067No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT2020.14465561936953067No Hit
AGTCAAAGGTGACAGAATTGGTCTTGTCTTTAGCCATTCCATGAGAGCCT2000.1432233855143868No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT2000.1432233855143868No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA1990.14250726858681487No Hit
GTCACTGTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGC1980.14179115165924291No Hit
GCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC1980.14179115165924291No Hit
CTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTT1950.1396428008765271No Hit
GTCAAAGGTGACAGAATTGGTCTTGTCTTTAGCCATTCCATGAGAGCCTC1950.1396428008765271No Hit
GTGTAATCTTCAACCGATTAGAGACATTGATACTATTAAGGGCTTTCACT1940.1389266839489552No Hit
ATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCC1930.13821056702138326No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1920.1374944500938113No Hit
ATTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT1920.1374944500938113No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC1920.1374944500938113No Hit
TTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTG1910.13677833316623939No Hit
CCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAATGGGAGTGCAGATGC1900.13606221623866746No Hit
AATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGA1870.13391386545595166No Hit
CACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGC1870.13391386545595166No Hit
GGCCCACATGATGAACCCCAACAAAGCAACACAAAGCAAAAAACATGATA1830.1310493977456639No Hit
CATCCGGAGTGGAGTCCGCCGTCTTGAGAGGGTTCCTCATTATAGGTAAA1820.13033328081809198No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATCCTTTTGGTCG1820.13033328081809198No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG1810.12961716389052005No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC1800.1289010469629481No Hit
ATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTT1790.12818493003537618No Hit
ATAATGAGGAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCTTTC1790.12818493003537618No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT1770.1267526961802323No Hit
ATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAA1730.12388822846994456No Hit
GAAGATACGGACCAGCATTAAGCATCAATGAACTGAGTAACCTTGCAAAA1720.12317211154237265No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC1720.12317211154237265No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCGCCGGCACT1720.12317211154237265No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1710.1224559946148007No Hit
GTAACCTTGCAAAAGGGGAAAAGGCTAATGTGCTAATTGGGCAAGGAGAC1680.12030764383208489No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA1680.12030764383208489No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATA1680.12030764383208489No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT1680.12030764383208489No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT1650.11815929304936909No Hit
TTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTG1640.11744317612179718No Hit
GGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCC1630.11672705919422524No Hit
AGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTG1630.11672705919422524No Hit
TCCGGAGTGGAGTCCGCCGTCTTGAGAGGGTTCCTCATTATAGGTAAAGA1620.11601094226665329No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT1620.11601094226665329No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA1620.11601094226665329No Hit
GTGGCGAGAGCAATTGGGACAGAAATTTGAGGAAATAAGATGGTTAATTG1610.11529482533908138No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG1610.11529482533908138No Hit
GTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAATGGAATGGTAA1610.11529482533908138No Hit
ATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCAACACA1600.11457870841150944No Hit
GCATTAAGCATCAATGAACTGAGTAACCTTGCAAAAGGGGAAAAGGCTAA1600.11457870841150944No Hit
CCTAACGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCAA1600.11457870841150944No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT1590.11386259148393749No Hit
GGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGCGTCTACGCTGCAGT1580.11314647455636558No Hit
GAAAAACACAGATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAA1570.11243035762879364No Hit
ATCCCCTTAGTCAAAGGTGACAAGATTGGTCTTGTCTTTAGCCATTCCAT1540.11028200684607783No Hit
GCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACC1530.10956588991850588No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1510.10813365606336203No Hit
ATTCAGTATTAATTGATGGCCATCCGAATCCTTTTGGTCGCTGTCTGGCT1510.10813365606336203No Hit
CCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGT1500.10741753913579008No Hit
CTCCAACTCTATGCTGACAAAATGACTGCCGTCAGCATCCACAGCATTCT1480.10598530528064623No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC1470.10526918835307428No Hit
TATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTAT1460.10455307142550237No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG1450.10383695449793043No Hit
CTTCCAGGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCAT1430.10240472064278656No Hit
GACTGGAAAGTGTCTTTGCAGGAAAGAACACAGATCTTGAGGCTCTCATG1420.10168860371521463No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT1420.10168860371521463No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG1410.10097248678764267No Hit
TAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGC1400.10025636986007076No Hit
CACATGATGAACCCCAACAAAGCAACACAAAGCAAAAAACATGATATGGC1400.10025636986007076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2450.0180.000021
AAGCAGG12850.0142.04287
GCAGGGT11250.0141.555549
GAGCAAA9950.0140.351761
CAAAAGC9800.0140.000024
TAGAAAC4800.0137.81252
AGCAGGG12050.0132.157688
GTAGAAA5150.0128.446611
CGAAAGA302.4028281E-4122.5245
CTAATAA501.0106487E-7122.59
GCGAAAG4150.0121.024092
AGCGAAA4150.0121.024091
CGAAAGC4400.0116.9318163
AGCAGGT1900.0116.052638
AAAAGCA12100.0113.388435
AAGGTCG1000.0110.259
GCAGGTC801.2551027E-10107.187519
AAAGCAG16850.0106.869446
AGAAACA6200.0106.693563
CACGGAG354.437057E-4105.0245
Produced by FastQC (version 0.11.2)