FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_gv1208.3520000005a1a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_gv1208.3520000005a1a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190188
Sequences flagged as poor quality0
Sequence length251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA59913.1500410120512337No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA35301.8560582160809307No Hit
GAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA25911.3623362146928302No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC23031.2109071024460007No Hit
AGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT21051.1067995877763055No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT19311.015311165793846No Hit
GCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC16270.8554693250888595No Hit
GTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGC12980.6824825961680022No Hit
AGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT11800.6204387237890929No Hit
ACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCA10460.5499821229520264No Hit
GGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTT9100.4784739310576903No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG8890.467432224956359No Hit
GATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGT8480.4458746082823312No Hit
AGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCA8320.43746187982417395No Hit
CATTTAGGGCATTTTGGATAAAGCGTCTACGCTGCAGTCCTCGCTCACTG7970.41905903632195507No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC7730.40643994363471936No Hit
GATTACTGATCGTCTTTTTTTCAAATGTATTTATCGTCGCTTTAAATACG7270.3822533493175174No Hit
CCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCAA7140.3754180074452647No Hit
CTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATG7130.37489221191662986No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG6930.3643763013439334No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT6780.35648936841441103No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAT6780.35648936841441103No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG6530.34334448019854036No Hit
ATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAAGA6410.3370349338549225No Hit
GTTTCTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT6280.3301995919826698No Hit
ATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAAAG6280.3301995919826698No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA6170.32441584116768674No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT6000.31547731718089467No Hit
TGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAAC5730.3012808379077544No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA5710.3002292468504848No Hit
CAGCATAGGTCTCACAGACAAATGGCTACTACCACCAATCCACTAATCAG5340.28077481229099627No Hit
TTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTT5280.2776200391191873No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT5200.2734136748901087No Hit
AATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCA5010.2634235598460471No Hit
CCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAATGGGAGTGCAGATGC4890.2571140135024292No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC4740.24922708057290682No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG4670.24554651187246304No Hit
GCAGAGGCCATGGAGGTTGCTAATCAGACTAGGCAGATGGTACATGCAAT4650.24449492081519336No Hit
ATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTT4380.23029844154205314No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA4360.22924685048478347No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC4330.227669463898879No Hit
GTCAAAGGTGACAGAATTGGTCTTGTCTTTAGCCATTCCATGAGAGCCTC4270.22451469072707003No Hit
GAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTA4220.22188571308389593No Hit
ATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAA4220.22188571308389593No Hit
CATTTAGGGCATTTTGGACAAAGCGTCTACGCTGCAGTCCTCGCTCACTG4210.22135991755526113No Hit
CCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAG4180.21978253096935663No Hit
AGTCAAAGGTGACAGAATTGGTCTTGTCTTTAGCCATTCCATGAGAGCCT4130.2171535533261825No Hit
TAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGC4080.2145245756830084No Hit
CTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAACAGGCAGCA4030.2118955980398343No Hit
GGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTG3890.20453446063894676No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTATCCAAAATGCC3710.1950701411235199No Hit
GACTTGAAGATGTCTTTGCAGGAAAGAACACAGATCTTGAGGCTCTCATG3660.19244116348034576No Hit
CGTACGTTCTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCG3630.19086377689444128No Hit
CAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGAT3630.19086377689444128No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG3590.18876059477990198No Hit
ATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCATCATGTGGGCCT3560.1871832081939975No Hit
CTTCCTTCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG3550.1866574126653627No Hit
TCTTAAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTC3340.1756157065640314No Hit
ATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGCTCCTG3270.1719351378635876No Hit
AAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATG3240.17035775127768313No Hit
GTGTTGCTTTGTTGGGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATT3240.17035775127768313No Hit
TCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCAT3150.16562559151996972No Hit
TGTTTCTACTAATAACCCGAGTAGAAACAAGGGTGTTTTTAATTAATGCA3150.16562559151996972No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG3060.1608934317622563No Hit
CACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGC3060.1608934317622563No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA3020.15879024964771699No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC2990.15721286306181254No Hit
TACAGCCCCCATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCAC2990.15721286306181254No Hit
GCTTTGTCCATGTTATTTGGGTCTCCATTCCCATTGAGGGCATTTTGGAC2950.15510968094727323No Hit
GCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC2940.1545838854186384No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA2930.15405808989000358No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC2920.15353229436136875No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT2880.15142911224682945No Hit
AATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGA2870.15090331671819462No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC2860.1503775211895598No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT2860.1503775211895598No Hit
ATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAA2830.14880013460365532No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT2820.14827433907502052No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG2800.14722274801775087No Hit
ATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCC2770.1456453614318464No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC2740.14406797484594192No Hit
GGCCCACATGATGAACCCCAACAAAGCAACACAAAGCAAAAAACATGATA2730.1435421793173071No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT2710.14249058826003744No Hit
GAAAAACACAGATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAA2660.13986161061686334No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT2650.13933581508822848No Hit
ATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGG2630.13828422403095883No Hit
GAAATAGCTCTCAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCT2630.13828422403095883No Hit
AGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACC2620.137758428502324No Hit
CATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAG2600.13670683744505435No Hit
CTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTT2600.13670683744505435No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA2580.13565524638778473No Hit
TTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTG2550.13407785980188025No Hit
ATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTT2540.13355206427324542No Hit
GGTCCCCATTCCCATTTAGGGCATTTTGGATAAAGCGTCTACGCTGCAGT2520.13250047321597577No Hit
AGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTG2520.13250047321597577No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT2520.13250047321597577No Hit
GCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCATCATGTG2510.13197467768734095No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC2490.1309230866300713No Hit
GATTCGCTTGGAGAAGCTGTGATGAGAATGGGAGACCTTCACTACCTCCA2480.13039729110143647No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA2450.12881990451553202No Hit
TATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTAT2440.12829410898689717No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG2390.12566513134372306No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2370.12461354028645341No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGATAAAGC2360.12408774475781857No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC2340.12303615370054892No Hit
CTTGAGTGGAATGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATT2310.12145876711464447No Hit
GTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAG2310.12145876711464447No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT2310.12145876711464447No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC2260.11882978947147034No Hit
ATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCAACACA2260.11882978947147034No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG2250.11830399394283551No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC2250.11830399394283551No Hit
ACCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCACCGTGCCCA2240.1177781984142007No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATAGGTC2240.1177781984142007No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCGCCGGCA2240.1177781984142007No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG2220.11672660735693102No Hit
GTCACTGTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGC2200.11567501629966138No Hit
GGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCC2200.11567501629966138No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG2170.11409762971375692No Hit
GGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGCGTCTACGCTGCAGT2150.11304603865648725No Hit
CCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGT2120.1114686520705828No Hit
AGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTG2090.10989126548467831No Hit
GACTGGAAAGTGTCTTTGCAGGAAAGAACACAGATCTTGAGGCTCTCATG2070.10883967442740868No Hit
GGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAAT2070.10883967442740868No Hit
AAATGGGAATGGGGACCCGAACAACATGGATAGAGCAGTTAAACTATACA2060.10831387889877384No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT2040.1072622878415042No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT2030.10673649231286936No Hit
TATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGT2030.10673649231286936No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC2020.10621069678423455No Hit
GCATGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGCCTCTGCTGC2020.10621069678423455No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC2010.10568490125559972No Hit
GGCATGAAAACAGAATGGTGCTGGCTAGCACTACGGCAAAGGCTATGGAA2000.10515910572696491No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG2000.10515910572696491No Hit
ACATCCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTC1960.10305592361242559No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG1960.10305592361242559No Hit
TTGTTGGGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATTAGGTGCAA1950.10253012808379078No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG1940.10200433255515594No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCCG100.0014752357245.00002245
GAGCGAA3550.0234.647871
TTCTAAT550.0200.454543
AATTTGG204.7800666E-5183.75002245
TTTCTAA600.0183.752
GAGCAAA12550.0165.936251
TCTAATT700.0157.54
CTAATTA700.0157.55
AAGCAGG20000.0144.549997
AGCAGGG16600.0143.162648
GCAGGGT16850.0141.038579
GCAGGTA2700.0136.111119
AGCGAAA6500.0128.153852
AGCAGGT3750.0124.133338
CGAAAGC6850.0119.817524
GCGAAAG6850.0119.817523
GCAGGTC851.8189894E-12115.294118
CAGGTCA901.8189894E-12108.888899
ATTAGTA1700.0108.0882341
GATGTCT3750.0107.7999959
Produced by FastQC (version 0.11.2)