FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_a39rep.3520000005a560.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_a39rep.3520000005a560.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130531
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA7800.5975592004964339No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT5660.4336134711294635No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT4310.33018976335123457No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG4240.3248270525775486No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC3660.2803931633098651No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT2780.2129762278692418No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCGTGGAACGTTATCTCCCTCTT2440.18692877553991005No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2190.16777623706246025No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2070.15858301859328433No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT1960.15015590166320644No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA1870.1432609878113245No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC1810.13866437857673655No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG1800.13789827703763857No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1770.1355999724203446No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1760.1348338708812466No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1750.1340677693421486No Hit
CGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAAGATG1710.13100336318575664No Hit
GTTCCCTGTCCAAGGGCAAATTGATAACACTTGTCAGGATCGCATGACAC1680.12870505856846265No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG1640.1256406524120707No Hit
CTTCCTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG1550.11874573856018877No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT1550.11874573856018877No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1500.1149152308646988No Hit
GCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTTGA1430.10955252009101285No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT1430.10955252009101285No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1410.10802031701281688No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT1380.10572201239552291No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT1380.10572201239552291No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1350.10342370777822892No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1320.10112540316093495No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAG100.001474707244.99998245
GCAGGGG1000.0196.09
GCAGGGT1200.0163.333349
CGAAAGC1550.0126.451614
AAGCAGG8750.0120.3999947
GCAAAAG7750.0110.6451643
CAAAAGC8200.0107.5609744
AGCAAAA8050.0106.521742
AGCAGGT5400.0102.083338
AGCAGGG3950.099.240518
GAGCAAA8950.094.4413451
GAGCGAA2150.091.1627961
TGATGCG552.390547E-589.090919
AAAGCAG14050.076.7259756
TACGGAT655.4740078E-575.384612
TCTATGC652.3103712E-775.384613
AAAAGCA11950.074.832645
GCGAAAG2650.073.9622653
AGCGAAA2700.072.592592
GCAGGTA5350.070.981319
Produced by FastQC (version 0.11.2)