FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_95.3520000005a48b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_95.3520000005a48b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193440
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTAACCGTACCAACCATCCACCATTCCCTCCCAACCATTTTCTATGA6530.33757237386269645No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC5470.2827750206782465No Hit
GCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAAC4890.25279156327543423No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT4590.2372828784119107No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG4340.22435897435897434No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA4130.21350289495450786No Hit
GTACAAATTGCTTCAAATGAGAACATGGATAATATGGGATCGAGCACTCT4050.20936724565756823No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT4040.20885028949545079No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG3550.1835194375516956No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC3470.17938378825475598No Hit
GCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGC3430.1773159636062862No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3370.17421422663358146No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG3290.17007857733664183No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCC3100.16025641025641024No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3060.15818858560794044No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2910.15043424317617865No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2770.14319685690653433No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2720.14061207609594706No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2670.13802729528535979No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2640.13647642679900746No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2630.13595947063689No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA2620.13544251447477254No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2610.1349255583126551No Hit
GTCGTACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCA2490.12872208436724564No Hit
GGATCAAGTGAGCAGGCAGCGGAGGCCATGGAGATTGCTAGTCAGGCCAG2430.12562034739454095No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2370.12251861042183623No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG2320.11993382961124896No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG2300.11889991728701406No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT2280.11786600496277917No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2260.11683209263854426No Hit
GGAGTGGAGGAAACACTAATCAACAGAGGGCCTCCGCAGGCCAAACCAGT2210.114247311827957No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA2200.11373035566583954No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA2170.11217948717948717No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC2140.11062861869313481No Hit
GGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAG2130.11011166253101737No Hit
GATTCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATT2130.11011166253101737No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2090.10804383788254755No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2080.10752688172043011No Hit
GTTCAAGAGTGCTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT2040.10545905707196031No Hit
TCCCTGTACACATTGTGGTCATAAGTTCCATTTCTTATTGATCCTATGCA2040.10545905707196031No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT2010.10390818858560794No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA1970.10184036393713813No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT1970.10184036393713813No Hit
GGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCAGCGGAGGCCATGG1960.10132340777502069No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA1960.10132340777502069No Hit
GTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTTCTGCATTGTC1940.10028949545078578No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGT550.0178.181825
GTAACCG550.0178.181824
GAACGCA150.0049637873163.33333245
AGGGTGT900.0149.722216
CTTACGG251.16324096E-4147.0245
CGAAAGC602.0954758E-9122.53
GGGTTAA450.001205309581.6666641
ACAAGGT1600.076.56257
TAGAAAC2900.076.0344852
GCAGGGT655.4806897E-575.384619
CATCTAA500.001831106873.5245
TACGGAT550.002672205466.8181842
CCGACGC550.002672205466.818184245
TCCAACT1701.0913936E-1164.852942
CCAACTC1701.0913936E-1164.852943
GTATTAA1151.9141407E-763.913041
ACTATTC801.5321406E-461.2500048
GGTCGTT403.74495E-461.2500047
TACATTG1604.4019544E-1061.2500046
CAGTACG600.003772329361.259
Produced by FastQC (version 0.11.2)