FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_89.3520000005a404.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_89.3520000005a404.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194532
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA7870.404560689243929No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC7610.3911952789258323No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC7580.3896531161968211No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG7480.3845125737667839No Hit
CCTGTAACCGTACCAACCATCCACCATTCCTTCCCAACCATTTTCTATGA6320.32488228157835214No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA6280.3228260646063373No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG5640.28992659305409907No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC5310.2729628030349763No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC5170.26576604363292416No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG4850.24931630785680506No Hit
GCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAAC4690.24109143996874552No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC4490.23081035510867107No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4390.22566981267863384No Hit
GCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGC4160.21384656508954825No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCC4100.21076223963152593No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT3760.1932843953693994No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC3380.17375033413525795No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3260.16758168321921327No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT3240.16655357473320587No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG3190.16398330351818724No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC3190.16398330351818724No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3180.1634692492751835No Hit
GTGATTTTTGGCCGACTAGAGACCATAGTATTACTAAGGGCTTTCACCGA3110.15987086957415747No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT3070.15781465260214259No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT2910.14958978471408302No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2900.1490757304710793No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2870.14753356774206813No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2830.14547735077005325No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA2770.14239302531203094No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA2690.13828059136800117No Hit
ATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAATG2660.13673842863898997No Hit
GTACAAATTGCTTCAAATGAGAACATGGATAATATGGGATCGAGCACTCT2630.13519626590997882No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA2630.13519626590997882No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA2620.1346822116669751No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2580.1326259946949602No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2570.13211194045195648No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC2510.12902761499393417No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2490.1279995065079267No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2480.12748545226492297No Hit
ATAATGAGGAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCTTTC2340.12028869286287089No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG2320.11926058437686345No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT2310.11874653013385973No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2290.11771842164785228No Hit
CTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGTTATTT2290.11771842164785228No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT2290.11771842164785228No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG2260.11617625891884112No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2230.11463409618982995No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA2220.11412004194682622No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2220.11412004194682622No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2170.11154977073180763No Hit
ATCTGATCCAGCATCACTCATTTTACTCCATAGAGTTTGTCCTTGTTCAT2130.10949355375979274No Hit
GGTCAAAATGAAATGGGGAATGGAGATGAGACGTTGCCTCCTTCAGTCAC2130.10949355375979274No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2110.10846544527378528No Hit
GGCAATCATGGAGAAAAACATCATGTTAAAAGCGAATTTCAATGTGATTT2110.10846544527378528No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2100.10795139103078157No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATA2080.10692328254477412No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC2080.10692328254477412No Hit
GGATCAAGTGAGCAGGCAGCGGAGGCCATGGAGATTGCTAGTCAGGCCAG2050.10538111981576297No Hit
AGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG2030.10435301132975551No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT2010.10332490284374807No Hit
GATTCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATT1990.10229679435774064No Hit
GCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACC1950.10024057738572574No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGT550.0200.454545
GTAACCG550.0200.454544
GCAGGGT2450.0140.09
CGAAAGC1800.0129.305543
ATTGTCT900.0122.499998
CAAAAGC7000.0118.999994
CGTACTC354.4397108E-4105.03
AGGGTGT1650.0103.939396
GGACAGT501.4912335E-597.999996
AGCAGGG4000.097.999998
AAGCAGG10450.090.263157
CAGTACG552.3935096E-589.090919
TTGTCTC1250.088.2000059
AGCGAAA2650.087.8301851
GCGAAAG2850.081.6666642
GTCGTAC450.001205319981.6666641
TCGTACT450.001205319981.6666642
CCGACGC450.001205319981.666664245
GCAAAAG10000.080.8500063
AGCAGGT6300.079.722238
Produced by FastQC (version 0.11.2)