FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_80.3520000005a3f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_80.3520000005a3f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174890
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGCCCTTCTTTTTAATTTGCCTCTCTCTGCATCTTTGGTCATCGT4250.2430098919320716No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC4210.24072274000800503No Hit
CCTGTAACCGTACCAACCATCCACCATTCCCTCCCAACCATTTTCTATGA4170.23843558808393847No Hit
GCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAAC4140.23672022414088856No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG3600.20584367316599006No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3450.1972668534507405No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3210.18354394190634113No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3190.18240036594430786No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCC3120.1783978500771914No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2890.16524672651380867No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2850.16295957458974214No Hit
GTACAAATTGCTTCAAATGAGAACATGGATAATATGGGATCGAGCACTCT2700.15438275487449254No Hit
GCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGC2670.1526673909314426No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2510.1435187832351764No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2430.13894447938704327No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2420.13837269140602665No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2400.13722911544399335No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2390.13665732746297674No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2390.13665732746297674No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2370.13551375150094347No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG2370.13551375150094347No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2360.13494196351992682No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGAAATCTAATGTCGCA2340.13379838755789353No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2210.12636514380467723No Hit
GGTCAAAATGAAATGGGGAATGGAGATGAGACGTTGCCTCCTTCAGTCAC2210.12636514380467723No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2140.12236262793756075No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2070.11836011207044428No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2030.11607296014637773No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA2020.11550117216536109No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG1990.11378580822231116No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC1950.11149865629824461No Hit
CTTCCTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG1910.10921150437417806No Hit
GTCGTACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCA1870.10692435245011149No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT1860.10635256446909487No Hit
GGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAG1780.10177826062096175No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1750.10006289667791182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACCG750.0179.666674
TAACCGT800.0168.43755
GGGTGAC301.1750981E-6163.333331
AAAAGCC1100.0122.53
GCAGGGG407.5480214E-491.875019
AAGCCCT1700.086.470595
GTCGTAC450.001205114981.6666641
AATTCTA450.001205114981.6666645
AGCGAAA1650.081.6666641
TCGTACT450.001205114981.6666642
AAAGCCC1850.079.459464
GCTATAA1101.5734258E-977.9545441
GCGAAAG1900.077.368422
TCGGTTG500.001830811873.5245
CCAACTC1359.640644E-1172.592593
TAGACAG1700.072.058825
TTAGACA1700.072.058824
TAACTAT1203.1341187E-971.4583365
CAGAACC1900.070.921069
AACCGTA1950.069.102566
Produced by FastQC (version 0.11.2)