FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_76.3520000005a4db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_76.3520000005a4db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80895
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA4070.5031213301192905No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCCTT2420.29915322331417266No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAGTCATGG1820.22498300265776625No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA1490.1841893812967427No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG1190.14710427096853948No Hit
CTTCCTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG1190.14710427096853948No Hit
GTTTAGTGTTGTTCCCTGTCCAAGGGCAAATTGATAACACTTGTCAGGAT1090.13474256752580507No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1090.13474256752580507No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT1030.12732554546016442No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1020.12608937511589097No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG1010.12485320477161753No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC990.12238086408307065No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT990.12238086408307065No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT990.12238086408307065No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT970.11990852339452376No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA970.11990852339452376No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG960.11867235305025033No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT960.11867235305025033No Hit
GTTCAGTGTTGTTCCCTGTCCAAGGGCAAATTGATAACACTTGTCAGGAT950.11743618270597689No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT940.11620001236170344No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC930.11496384201743No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTTCCCGCGCTGGGGT930.11496384201743No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA920.11372767167315656No Hit
GTATATGGGCCCCCCAGTTTTCTTGGGATCTTTTCCCGCGCTGGGGTGTT910.11249150132888312No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA890.11001916064033623No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG870.10754681995178936No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA860.1063106496075159No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA850.10507447926324247No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA840.10383830891896903No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT840.10383830891896903No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT830.10260213857469559No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC820.10136596823042214No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG820.10136596823042214No Hit
GGTTTGGCCTGCGGAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGG810.1001297978861487No Hit
ACACTAAACAACGTGCATTCAAATAACACAGTACGTGATAGGACCCCTTA810.1001297978861487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGG700.0175.000029
CGAAAGC600.0163.333334
CTAAATT150.0049584764163.333338
GCTAAAT150.0049584764163.333337
GCAGGGT700.0140.000029
CAAAAGC3350.0117.014924
AGGGTGT458.804091E-6108.888896
AGCAAAA3750.0101.266662
GCAAAAG3900.097.3717963
AAGCAGG5050.094.603967
GAGCAAA4150.094.457831
GCGAAAG1051.0913936E-1193.333333
TACATCA407.533224E-491.875017
AGCAGGG2600.089.519238
GAGCGAA1101.6370905E-1189.090911
AGCAGGT2300.085.217398
TACATAC450.001202757281.666673
ACATCAC450.001202757281.666678
TTACATA450.001202757281.666672
TCTATGC450.001202757281.666673
Produced by FastQC (version 0.11.2)