FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_69.3520000005a23d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_69.3520000005a23d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126500
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA6490.5130434782608695No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC6400.5059288537549407No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT6250.4940711462450593No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA4950.391304347826087No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA4640.366798418972332No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT4590.36284584980237156No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA4380.34624505928853755No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC4270.3375494071146245No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG4260.3367588932806324No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC4000.31620553359683795No Hit
CTCCAACTCTATGCTGACAAAATGACTGCCGTCAGCATCCACAGCATTCT3740.2956521739130435No Hit
ACTCTATGCTGACAAAATGACTGCCGTCAGCATCCACAGCATTCTGCTGT3680.2909090909090909No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG3430.2711462450592885No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC3380.267193675889328No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT3060.24189723320158105No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3010.23794466403162054No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3000.23715415019762848No Hit
CTCTTTGTCCTTGTTCATTTCTCTCCGGAACCATTTCTGTTATCCTTTTG2970.23478260869565218No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2930.23162055335968382No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2880.2276679841897233No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA2700.21343873517786563No Hit
ATATACAACAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2640.20869565217391303No Hit
CCTGTAACCGTACCAACCATCCACCATTCCCTCCCAACCATTTTCTATGA2610.20632411067193676No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC2550.2015810276679842No Hit
GTACTGGAACTAGGATGAGTCCCAATGGTTCTCATTGCCTGCACCATCTG2380.18814229249011857No Hit
TCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGGGCCCTA2200.17391304347826086No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2190.17312252964426877No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG2170.1715415019762846No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2140.1691699604743083No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCT2040.16126482213438736No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2010.15889328063241107No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA2000.15810276679841898No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT2000.15810276679841898No Hit
TCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGGGCCCTA1890.14940711462450593No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA1840.14545454545454545No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1830.14466403162055336No Hit
CATATACAACAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1800.14229249011857706No Hit
TCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAA1790.141501976284585No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTT1770.1399209486166008No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTGTTGTATA1710.13517786561264822No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG1690.133596837944664No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC1660.13122529644268774No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC1660.13122529644268774No Hit
ATGGCTCTCATTGCCTGCACCATCTGCCTGGCCTGACTAGCAATCTCCAT1640.12964426877470356No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGCCGT1620.12806324110671935No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1580.12490118577075097No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC1570.1241106719367589No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1560.1233201581027668No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1560.1233201581027668No Hit
GTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA1520.12015810276679842No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1520.12015810276679842No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA1510.11936758893280631No Hit
CAACAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1490.11778656126482213No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1480.11699604743083003No Hit
GGGTACACTATGGACACAGTCAACAGAACACACCAATACTCAGAGAGGGG1480.11699604743083003No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG1470.11620553359683794No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1470.11620553359683794No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT1450.11462450592885376No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG1440.11383399209486166No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1420.11225296442687747No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT1400.11067193675889328No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT1380.1090909090909091No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA1370.108300395256917No Hit
GGCCTCCACCATGCTAGAAATTCCAATCGGTCTCCTATATGAACTACTAG1360.10750988142292489No Hit
GTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGTCAAGAAAACTGC1350.10671936758893281No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATAGTAGCACT1340.10592885375494071No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA1330.10513833992094862No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCC1320.10434782608695652No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT1310.10355731225296444No Hit
GTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTG1290.10197628458498023No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC1290.10197628458498023No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC1270.10039525691699605No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCC100.0014746536245.00002245
GCTCGGA100.0014746536245.000026
TAACCGT251.6061676E-9245.05
GTAACCG251.6061676E-9245.04
GAGCGAA1550.0181.77421
TTTGTAG150.0049617696163.333334
CGGAGAT150.0049617696163.333339
CGAAAGC2050.0137.439044
GCGAAAG2100.0134.166663
CAAAAGC6850.0132.335774
AGCGAAA2300.0122.500012
GCAGGGT1600.0122.500019
TCCTATT602.0881998E-9122.51
AAGCAGG9800.0118.750017
GCAGGGG458.823492E-6108.888899
GCAAAAG8450.0107.278113
TGTATCC354.4360835E-4105.04
TGTCGTA354.4360835E-4105.02
TCTATCT354.4360835E-4105.02
AGCAAAA8800.0103.011352
Produced by FastQC (version 0.11.2)