FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_56.3520000005a1f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_56.3520000005a1f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83727
Sequences flagged as poor quality0
Sequence length251
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3410.40727602804352236No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA3190.381000155266521No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2640.31531047332401735No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT2600.31053304191001707No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT2330.27828537986551527No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2300.2747023063050151No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT1820.21737312933701194No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA1670.199457761534511No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1400.1672100994900092No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA1380.16482138378300906No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA1370.163627025929509No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC1140.13615679529900748No Hit
CTGGGCACGGTGAGCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGG1140.13615679529900748No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT1110.1325737217385073No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1090.13018500603150715No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT950.11346399608250624No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT930.1110752803755061No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC910.10868656466850597No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG870.10390913325450571No Hit
GAGTTTAAGTTCAGTGCACATTTGGATGTAGAATCTCCCAATTCCATCAA840.1003260596940055No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCGTGGAACGTTATCTCCCTCTT840.1003260596940055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGG550.0200.454549
TTTAAAG204.77056E-5183.749982
ATCTATA150.004958787163.33333245
GGTCGTT150.004958787163.333337
CGTACTC150.004958787163.333333
AGGTCGT150.004958787163.333336
CGAAAGC900.0149.722234
AAGCAGG4450.0134.887637
GCAGGGT850.0129.705899
AGCAGGT2550.0115.294128
AGGGTTA458.8059205E-6108.888899
GCAAAAG4100.0107.5609743
AGCATCG354.4307832E-4105.07
CATCGCA354.4307832E-4105.09
GCATCGC354.4307832E-4105.08
AGCAAAA4500.098.02
AGCAGGG2400.091.8758
TTTAAAC407.5341633E-491.874993
CCTGAGC407.5341633E-491.874993
ACAAGGT407.5341633E-491.874993
Produced by FastQC (version 0.11.2)