FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_41.3520000005a4f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_41.3520000005a4f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79162
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA3050.38528586948283267No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA1960.247593542356181No Hit
CCTGTAACCGTACCAACCATCCACCATTCCCTCCCAACCATTTTCTATGA1920.24254061292034054No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT1800.22738182461281928No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT1770.22359212753593893No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1690.21348626866425807No Hit
GCCATTGGGACTCATCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCT1650.20843333922841767No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC1600.20211717743361715No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAACCACATTGAAATTCGCTT1510.19074808620297617No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1370.17306283317753468No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1360.17179960081857457No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1340.16927313610065436No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT1320.16674667138273414No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1310.16548343902377402No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG1310.16548343902377402No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG1280.1616937419468937No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTACAGACCTATCAGAAACGAAT1280.1616937419468937No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC1180.14906141835729264No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1130.1427452565624921No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA1130.1427452565624921No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC1120.141482024203532No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTACAGACCTATC1110.1402187918445719No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC1080.13642909476769158No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1080.13642909476769158No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA1050.13263939769081126No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA1040.13137616533185115No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1040.13137616533185115No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1020.12884970061393095No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA990.1250600035370506No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC990.1250600035370506No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC990.1250600035370506No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG980.1237967711780905No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG980.1237967711780905No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA970.12253353881913039No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT970.12253353881913039No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTACAGA950.12000707410121018No Hit
CCATTGGGACTCATCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTT930.11748060938328996No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA930.11748060938328996No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA920.11621737702432984No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA910.11495414466536975No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAACCACATTGAAATTCGCTTTT910.11495414466536975No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA900.11369091230640964No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG900.11369091230640964No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG890.11242767994744952No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG890.11242767994744952No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA880.11116444758848942No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG870.10990121522952932No Hit
GTACTGGTCTAAGAGATGATCTTCTTGAAAATTTACAGACCTATCAGAAA850.1073747505116091No Hit
GGATCAAGTGAGCAGGCAGCGGAGGCCATGGAGATTGCTAGTCAGGCCAG850.1073747505116091No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT850.1073747505116091No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC840.106111518152649No Hit
GTGGTTTTTGGCCGACTAGAGACCATAGTATTACTAAGGGCTTTCACCGA840.106111518152649No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC830.10484828579368889No Hit
GTACAAATTGCTTCAAATGAGAACATGGATAATATGGGATCGAGCACTCT810.10232182107576868No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG800.10105858871680858No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATAC150.0049582794163.33334245
GGCTACT150.0049582794163.333341
GTACTGA402.6569978E-8153.124986
TAACCGT251.16066476E-4147.05
GAGCGAA251.16066476E-4147.01
GTAACCG251.16066476E-4147.04
CGAAAGC601.2732926E-11142.916673
AGCGAAA601.2732926E-11142.916671
AGGTACT455.3680196E-8136.111114
CTAAATT302.3989337E-4122.500018
CAGTACG302.3989337E-4122.500014
GCTAAAT302.3989337E-4122.500017
AGGGTGT404.9015835E-6122.499996
TACTGAT551.776989E-7111.363637
CAAGCTA354.429883E-4104.999994
ACTGATT602.984707E-7102.083348
CAAAAGC3350.098.7313464
GCAGGGT801.535409E-891.874999
GCAAAAG3500.091.000013
ACAAGGG954.8748916E-1090.263153
Produced by FastQC (version 0.11.2)