FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_295.3520000005a0ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_295.3520000005a0ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159110
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT24011.5090189177298725No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC11670.7334548425617498No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA11030.6932310979825278No Hit
GCAATATGCACTCACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTT9530.5989566966249764No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC8790.5524479919552511No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA8360.5254226635660864No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC7590.47702847086920996No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA6870.43177675821758527No Hit
GCAATAAGTCAGTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGA6790.4267487901451826No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA6230.39155301363836337No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC5810.365156181258249No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA5710.3588712211677456No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG5590.35132926905914147No Hit
GTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA5120.32178995663377535No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA4920.3092200364527685No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG4700.295393124253661No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC4640.29162214819935894No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG4400.2765382439821507No Hit
GTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGTCAAGAAAACTGC4150.26082584375589213No Hit
GAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT4090.2570548677015901No Hit
ACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG3850.2419709634843819No Hit
ATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGT3810.2394569794481805No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT3620.22751555527622402No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3620.22751555527622402No Hit
GACTTAGCCATTGATGAAATGGAAATCTGAATACATGAAACACACCTCCA3540.22248758720382128No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT3510.2206020991766702No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3490.21934510715856956No Hit
AGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT3450.21683112312236819No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA3320.20866067500471372No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA3320.20866067500471372No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA3270.205518194959462No Hit
AGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT3230.20300421092326063No Hit
AGTAGAAACAAGGTACTTTTTTTGGACAGTACGGATAACAAATAGTAGCA3180.19986173087800896No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3030.19043429074225376No Hit
ATATACAATAGGATGGGGGCTATAACCACTGAAGTGGCATTTGGCCTGGT3010.1891772987241531No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGCTTGTCCCT2960.1860348186789014No Hit
GGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCAT2890.181635346615549No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2850.1791213625793476No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA2850.1791213625793476No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2820.17723587455219658No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2820.17723587455219658No Hit
GTAGAAACAAGGTACTTTTTTTGGACAGTACGGATAACAAATAGTAGCAC2730.17157941047074352No Hit
GACTTATTGCTGCAAATTTGTTGGTTTCAATTTTCAGATCCTCCCCATAC2730.17157941047074352No Hit
CAATATGCACTCACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTC2540.159637986298787No Hit
GTCCCTAAGAGCCTGAACAATAAGGAGCAGTTTTCTTGACTCTGCTGAAA2510.15775249827163598No Hit
ATATGCACTCACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCAT2490.15649550625353528No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGCTTGTCCCTAAGAG2460.15461001822638426No Hit
AGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGTCAAGAAA2460.15461001822638426No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2450.1539815222173339No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2400.1508390421720822No Hit
CAATAGGATGGGGGCTATAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2360.14832505813588082No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2360.14832505813588082No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT2360.14832505813588082No Hit
TTTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTA2350.1476965621268305No Hit
GAACAATAAGGAGCAGTTTTCTTGACTCTGCTGAAAATCCTTCCAATTGT2330.14643957010872982No Hit
GCAATGAAAGAGTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGC2270.14266859405442775No Hit
TCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGTCA2180.13701212997297466No Hit
GATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAA2150.13512664194582363No Hit
ATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCC2140.13449814593677328No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT2100.13198416190057194No Hit
CCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGTCAAGAAAAC2090.1313556658915216No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC2070.13009867387342092No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA2050.12884168185532022No Hit
CTTAGGGACAAGCTCGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA1980.12444220979196782No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG1980.12444220979196782No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC1970.12381371378291747No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTCA1920.12067123373766576No Hit
TGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAAACCCAGGGATCAT1920.12067123373766576No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG1910.12004273772861541No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1880.11815724970146439No Hit
CAATAAGTCAGTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGAT1880.11815724970146439No Hit
GCGAAAGAGTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGC1820.11438627364716233No Hit
ATACAATAGGATGGGGGCTATAACCACTGAAGTGGCATTTGGCCTGGTGT1800.11312928162906166No Hit
TTTCTTGACTCTGCTGAAAATCCTTCCAATTGTGGTGATGCATACAGGCT1780.11187228961096096No Hit
TTATTGTTCAGGCTCTTAGGGACAAGCTCGAACCTGGGACCTTTGATCTT1780.11187228961096096No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA1780.11187228961096096No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT1770.11124379360191064No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT1760.11061529759286029No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG1750.10998680158380994No Hit
GTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAAACCCAGGGATCATT1710.10747281754760858No Hit
GTCGTACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCA1710.10747281754760858No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1710.10747281754760858No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1660.10433033750235686No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1640.10307334548425616No Hit
AAACAAGGTACTTTTTTTGGACAGTACGGATAACAAATAGTAGCACTGCT1640.10307334548425616No Hit
CATATACAATAGGATGGGGGCTATAACCACTGAAGTGGCATTTGGCCTGG1640.10307334548425616No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT1630.10244484947520584No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1620.10181635346615549No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1620.10181635346615549No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGGG100.00147501245.00002245
ACCTCGC100.00147501245.00002245
AAGCAGG12150.0130.061727
CAAAAGC10550.0130.04744
AGCAGGT9800.0125.000018
TTGTGGG302.4034534E-4122.5245
CGAAAGC2300.0106.5217364
GCAGGGG354.438208E-4105.000019
GCAGGGT1750.0104.999999
GCAAAAG12950.0104.0540543
GCAGGTA8750.0103.69
GAGCGAA2500.093.11
AAAGCAG17350.091.786746
TAGAAAC3350.091.417912
GGGTATT552.3921653E-589.090917
AGGGTAT552.3921653E-589.090916
GCAGGTC3600.088.472229
TTTAAAC1300.084.8076863
CTATTCA1300.084.8076869
TAGTTTT903.4995537E-881.666679
Produced by FastQC (version 0.11.2)