FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_294.3520000005a0df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_294.3520000005a0df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173436
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT7700.44396780368550937No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA7490.4318595908577227No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA7410.42724693835189925No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT6950.4007241864434143No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA6760.3897691367420836No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG4910.28310154754491573No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC4770.2750294056597246No Hit
ATATACAATAGGATGGGGGCTATAACCACTGAAGTGGCATTTGGCCTGGT4570.263497774395166No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4390.25311930625706314No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA4290.2473534906247838No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG4160.23985793030282063No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3910.22544339122212229No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA3840.22140732027952675No Hit
GATGCAACGATTCAAGTGACCCGCTTGTTGCTGCCGCGAATATCATTGGG3820.22025415715307087No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT3710.2139117599575636No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT3570.20583961807237253No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT3570.20583961807237253No Hit
CAATAGGATGGGGGCTATAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3370.19430798680781383No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3180.18335293710648307No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT3130.18047002929034342No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3080.17758712147420375No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2990.17239788740515233No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG2950.17009156115224058No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT2930.16893839802578473No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2870.16547890864641712No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2780.1602896745773657No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT2780.1602896745773657No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC2740.157983348324454No Hit
GTCGTACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCA2730.15740676676122603No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG2710.15625360363477017No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT2620.15106436956571878No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2610.15048778800249082No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2560.14760488018635115No Hit
CATATACAATAGGATGGGGGCTATAACCACTGAAGTGGCATTTGGCCTGG2560.14760488018635115No Hit
GTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGTGTT2540.1464517170598953No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG2530.14587513549666736No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA2530.14587513549666736No Hit
GTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTG2500.14414539080698355No Hit
AGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT2490.14356880924375562No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT2490.14356880924375562No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2410.13895615673793216No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA2410.13895615673793216No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2390.13780299361147627No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2380.13722641204824834No Hit
CCTGTAACCGTACCAACCATCCACCATTCCCTCCCAACCATTTTCTATGA2360.13607324892179248No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC2320.13376692266888074No Hit
GTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGC2170.12511819922046172No Hit
ATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTTGACAG2150.12396503609400586No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2140.12338845453077792No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2090.12050554671463826No Hit
ATACAATAGGATGGGGGCTATAACCACTGAAGTGGCATTTGGCCTGGTGT2040.11762263889849858No Hit
GTACAAATTGCTTCAAATGAGAACATGGATAATATGGGATCGAGCACTCT2000.11531631264558684No Hit
GTTCAAGAGTGCTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT1960.11300998639267512No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCC1930.11128024170299129No Hit
CTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGGACCAGGAGTGGAGGA1920.11070366013976338No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1890.10897391545007958No Hit
ATTGTGGATTCTTGATCGTCTTTTTTTCAAATGCGTCTATCGACTCTTCA1870.1078207523236237No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA1860.10724417076039576No Hit
GGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTT1840.1060910076339399No Hit
GCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC1810.1043612629442561No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1810.1043612629442561No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA1810.1043612629442561No Hit
ACGATTCAAGTGACCCGCTTGTTGCTGCCGCGAATATCATTGGGATCTTG1790.10320809981780021No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG1790.10320809981780021No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC1780.1026315182545723No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT1780.1026315182545723No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTATAGCCCCCATCCTATTGTATA1750.1009017735648885No Hit
AGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCA1740.10032519200166055No Hit
AGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAA1740.10032519200166055No Hit
ACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCA1740.10032519200166055No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATA151.5171525E-5245.02
TAGGCAT151.5171525E-5245.09
CTCCCGC150.0049633477163.33333245
CCTGTAA150.0049633477163.333331
CGAATAA150.0049633477163.33333245
CGCAAAG150.0049633477163.33333245
GCAGGGG750.0147.09
GCAGGGT4500.0136.111119
CGAAAGC2300.0127.8260963
TATAGGC302.4038226E-4122.57
GCAAAAG8900.0119.747193
AGCAGGG6400.0112.929688
TTTAAAC1000.0110.253
AAGCAGG11150.0109.865477
CAAAAGC10800.098.680554
AGCAGGT4650.094.838718
ACTATTC1250.088.28
GCGAAAG3350.087.761192
AGCGAAA3400.086.470591
CTATTCA1300.084.8076869
Produced by FastQC (version 0.11.2)