FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_292.3520000005a171.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_292.3520000005a171.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97561
Sequences flagged as poor quality0
Sequence length251
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA8050.8251247937188017No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCCTT5190.5319748670062833No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4250.4356248910937772No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3200.3279999180000205No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2550.2613749346562663No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2440.25009993747501563No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1980.2029499492625127No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1960.20089994977501255No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT1900.19474995131251216No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1860.19064995233751192No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT1820.18654995336251165No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG1810.1855249536187616No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1770.18142495464376135No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT1760.18039995490001126No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1730.17732495566876108No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1700.1742499564375109No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1650.16912495771876057No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1610.16502495874376033No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1540.15784996053750988No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1480.15169996207500946No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1440.14759996310000922No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1380.14144996463750886No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1350.13837496540625865No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1320.13529996617500845No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1320.13529996617500845No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT1280.1311999672000082No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT1190.12197496950625764No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1170.11992497001875749No Hit
GCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCCTTGA1170.11992497001875749No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1150.11787497053125737No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1130.11582497104375723No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT1120.11479997130000717No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1120.11479997130000717No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1110.11377497155625711No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG1100.11274997181250704No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA1090.11172497206875698No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC1080.11069997232500692No Hit
TTCCATGAGAGCCTCAAGATCTGTGTTTTTCCCAGCAAAGACATCTTCAA1050.10762497309375672No Hit
GTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGTGTT1050.10762497309375672No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT980.10044997488750627No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT980.10044997488750627No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC980.10044997488750627No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGGG100.0014741374245.00002245
GCAGGGG750.0179.666679
GCGATTA150.0049600387163.33333245
CGACTGG150.0049600387163.33333245
TATAACC150.0049600387163.33333245
GCAGGGT1550.0158.064539
ACAAGGT455.3774784E-8136.111113
AGCAAAA5900.0124.576272
AGCAGGG3150.0124.444458
AAGCAGG6900.0124.275367
CGAAAGC900.0122.54
GCAAAAG6050.0121.48763
CAAAAGC6450.0113.95354
AGCAGGT3250.0109.307698
TAGTTTT458.813291E-6108.888899
GGTAGTT458.813291E-6108.888897
GAGCAAA7350.0101.666671
GCAGGTA3200.088.0468759
ACAAGGG707.487233E-787.57
TAGAAAC903.4853656E-881.666672
Produced by FastQC (version 0.11.2)