FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_291.3520000005a0c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_291.3520000005a0c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107371
Sequences flagged as poor quality0
Sequence length251
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT11361.058013802609643No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT8640.8046865540974751No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC7530.70130668430023No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA7310.6808169803764518No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA4420.41165677883227314No Hit
CTTCCTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG4160.38744167419508063No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA3750.3492563168825847No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA3440.32038446135362436No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG3350.31200230974844234No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA3300.30734555885667453No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG3020.28126775386277486No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC2920.2719542520792393No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC2870.2672975011874715No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT2770.25798399940393585No Hit
CTCTTTGTCCTTGTTCATTTCTCTCCGGAACCATTTCTGTTATCCTTTTG2560.23842564565851113No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2470.2300434940533291No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC2440.22724944351826845No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2280.21234784066461151No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2270.21141649048625794No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG2190.20396568905942944No Hit
GAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT2140.19930893816766165No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2120.19744623781095455No Hit
GTATTTTAAGTGGGTCAACCAATTTAATCTACTAAAGAAACTACTACTGG2020.18813273602741895No Hit
ATATACAACAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1890.1760251837088227No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1880.17509383353046912No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC1870.17416248335211554No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA1840.17136843281705488No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG1840.17136843281705488No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT1820.16950573246034775No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA1820.16950573246034775No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCT1740.16205493103351928No Hit
GATCTTATGATCTCAGAGAACTCTTCCTTCTTAATCCTTCCAGACTCGAA1720.16019223067681218No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT1690.1573981801417515No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT1630.15181007907163013No Hit
GGGTACACTATGGACACAGTCAACAGAACACACCAATACTCAGAGAGGGG1620.15087872889327658No Hit
GTACTGGAACTAGGATGAGTCCCAATGGTTCTCATTGCCTGCACCATCTG1620.15087872889327658No Hit
GATCTTCATGATCTCAGAGAACTCTTCCTTCTTAATCCTTCCAGACTCGA1590.1480846783582159No Hit
CTCCAACTCTATGCTGACAAAATGACTGCCGTCAGCATCCACAGCATTCT1560.14529062782315522No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1560.14529062782315522No Hit
CTTAAACTACACATACCCAGCATTGAACGTGACTATGCCAAACAATGAAC1530.14249657728809456No Hit
TCCTCAAGAATTCCCCTTTGGCTTGTGTTTAGAATAGAGCGGTTCCTTTT1520.14156522710974098No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT1510.14063387693138743No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1490.13877117657468033No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCC1480.13783982639632675No Hit
TAGTACCCTGTCCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGG1450.13504577586126607No Hit
CCCTTACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACACT1450.13504577586126607No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA1440.1341144256829125No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG1420.13225172532620538No Hit
ACTCTATGCTGACAAAATGACTGCCGTCAGCATCCACAGCATTCTGCTGT1400.13038902496949828No Hit
AGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT1390.12945767479114473No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1350.1257322740777305No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1340.12480092389937693No Hit
AGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG1330.12386957372102338No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1290.12014417300760913No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1240.11548742211584134No Hit
GAGCAGTTTTCTTGACTCTGCTGAAAATCCTTCCAATTGTGGTGATGCAT1240.11548742211584134No Hit
CTCTATCTGATCCAGCATCACTCATTTTACTCCATAGAGTTTGTCCTTGT1240.11548742211584134No Hit
GACTTATTGATTCGCCTTGTTCATTGATGAAATGGAAATCTGAATACATG1200.1117620214024271No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTT1200.1117620214024271No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT1190.11083067122407354No Hit
GTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA1190.11083067122407354No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1180.10989932104571998No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA1170.10896797086736641No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1170.10896797086736641No Hit
AGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT1160.10803662068901286No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG1130.10524257015395219No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1080.10058581926218439No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGT1100.0178.181829
CAAAAGC7950.0155.628924
GCAAAAG8450.0147.869833
AGCAGGG1850.0145.675678
AAGCAGG10550.0142.81997
CGAAAGC1850.0139.054064
GCGAAAG1850.0139.054063
GCAGGGG551.1386874E-9133.636379
AGCAAAA9500.0132.81582
GCAGGTA4900.0125.000019
AAAGCAG12250.0123.06
AGCAGGT7200.0119.097238
GAGCAAA10500.0119.01
GAGCGAA2100.0116.6666641
CAAGCTA757.4578566E-11114.3333364
TAGTACC706.0936145E-9105.01
AAAAGCA12000.0104.1255
GCAGGTC2600.094.230779
CTAAATT801.540684E-891.875018
GTCGTTT407.5400167E-491.875018
Produced by FastQC (version 0.11.2)