FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_289.3520000005a164.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_289.3520000005a164.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166245
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA23431.4093656952088784No Hit
GTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGC17371.0448434539384643No Hit
AGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT15420.927546693133628No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC15280.9191253872296912No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT11540.694156215224518No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA9820.5906944569761496No Hit
GATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGT8530.5130981382898734No Hit
GGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTT7840.47159313062046976No Hit
ACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCA6500.3909892026827875No Hit
CTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATG5320.3200096243496045No Hit
GGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTG5250.315798971397636No Hit
AGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCA5130.3085807091942615No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5000.30076092514060576No Hit
TCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCAT4550.2736924418779512No Hit
CCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCAA4430.2664741796745767No Hit
AAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATG4350.261662004872327No Hit
TGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAAC4130.24842852416614034No Hit
ATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCATCATGTGGGCCT3930.23639808716051613No Hit
AGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT3840.2309843905079852No Hit
GCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC3790.22797678125657914No Hit
AATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCA3760.22617221570573554No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC3730.22436765015489188No Hit
GAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA3520.21173569129898642No Hit
GTGTTGCTTTGTTGGGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATT3500.21053264759842402No Hit
ATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTT3480.2093296038978616No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3450.20752503834701794No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3390.20391590724533068No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3300.19850221059279977No Hit
TTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTT3250.19549460134139374No Hit
ATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAA3010.18105807693464465No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3000.18045655508436342No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2900.17444133658155134No Hit
TAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGC2890.1738398147312701No Hit
ATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGG2700.1624108995759271No Hit
CAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGAT2680.16120785587536468No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTTCC2670.16060633402508348No Hit
ATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTT2560.15398959367199014No Hit
AGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTG2540.1527865499714277No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2520.15158350627086528No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2430.1461698096183344No Hit
TTGTTGGGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATTAGGTGCAA2390.14376372221720954No Hit
TGTTTCTACTAATAACCCGAGTAGAAACAAGGGTGTTTTTAATTAATGCA2380.1431622003669283No Hit
GCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC2370.1425606785166471No Hit
ATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAA2270.136545460013835No Hit
GAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTA2260.1359439381635538No Hit
CCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGT2170.13053024151102288No Hit
CTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTT2160.12992871966074168No Hit
ATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCC2160.12992871966074168No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2130.12812415410989803No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA2010.1209058919065235No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT1980.11910132635567987No Hit
GCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCATCATGTG1970.11849980450539867No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1940.11669523895455503No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1910.1148906734037114No Hit
CACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGC1900.11428915155343017No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT1900.11428915155343017No Hit
ATGTGGGCCTGCCAAAAGGGCAACATTAGGTGCAACATTTGCATTTGAGT1880.11308610785286777No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1830.1100784986014617No Hit
AATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGA1820.1094769767511805No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1820.1094769767511805No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCCATCGTTCCATCAGGCCCC1800.10827393305061807No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1690.10165719269752474No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC3550.0138.028173
GCGAAAG3700.0132.432432
AGCGAAA4650.0105.376341
AGGGTGT6500.098.06
CAAGGGT6900.094.09424
GCAAAAG7600.093.486843
AAGGGTG6950.093.417275
GGTGTTT7050.090.354618
ACAAGGG7200.090.1736153
CAAAAGC8500.083.5882344
TTGGAGG903.500645E-881.666674
CGTACTC450.001205009281.666673
TAGAAAC6350.079.094492
GCAGGGT6750.078.037038
ACTTGGA955.0928065E-877.3684162
GAGCAAA9450.076.481481
GGGTGTT8550.075.935677
TCCAACT1306.91216E-1175.384612
AAGCAGG11350.074.471367
GTCGTAC500.001830651873.51
Produced by FastQC (version 0.11.2)