FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_286.3520000005a33c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_286.3520000005a33c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199879
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC16600.8305024539846608No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA15990.79998399031414No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT15590.7799718829892085No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA7870.3937382116180289No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC7190.3597176291656452No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG7150.35771641843315205No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA6560.328198560128878No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA5390.2696631462034531No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT5290.2646601193722202No Hit
CTTCCTTCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG5100.2551543683928777No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA5000.25015134156164476No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG4950.24764982814602837No Hit
ACTTTAATTGAAGACCCAGATGAAAGCACATCCGGAGTGGAGTCCGCCGT4910.2456486174135352No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC4800.240145287899179No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG4800.240145287899179No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT4790.2396449852160557No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA4770.23864437984980913No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC4490.22463590472235703No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC4370.21863227252487757No Hit
GTATAGGAAACTTAAAAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG4310.2156304564261378No Hit
GTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTTAGAATAGAGCGGTTC4220.2111277322780282No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT4040.202122283981809No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA4000.20012107324931583No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC3960.19811986251682268No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG3900.19511804641808295No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG3840.19211623031934322No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC3770.18861411153748017No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3720.18611259812186373No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3710.18561229543874044No Hit
ATAATGAGGAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCTTTC3640.1821101766568774No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3500.17510593909315136No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3420.17110351762816503No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC3420.17110351762816503No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA3380.16910230689567188No Hit
GTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA3350.167601398846302No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA3330.16660079348005544No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG3300.16509988543068557No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG3230.16159776664882253No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC3120.15609443713446636No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2980.1490901995707403No Hit
GAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT2940.14708898883824714No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG2840.14208596200701423No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2780.1390841459082745No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA2780.1390841459082745No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG2660.13308051371079502No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT2660.13308051371079502No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2640.13207990834454847No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTACC2610.13057900029517858No Hit
AGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG2600.1300786976120553No Hit
ATTTCATTGCCATCATCCATTTCATCCTAAGTGACGGGTTCTTTTCCTGT2570.12857778956268542No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC2550.12757718419643885No Hit
GTTAAAGCACAGATTTGAAATAATCGAGGGGAGAGACAGAACAATGGCCT2540.12707688151331556No Hit
ATTGAATACTGACTTAGCCAATAGAGTCCTACAGACTTTCCCAATGGAAC2460.12307446004832924No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2460.12307446004832924No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC2420.12107324931583609No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA2410.1205729466327128No Hit
CTTTTAAGGCCGTGTTTGAAAAGTCGATAGACGCATTTGAAAAAAAGACG2400.1200726439495895No Hit
GAGCAAAAGCAGGAGTGAAGATGAATCCAAATCAAAAGATAATAACGATT2310.1155699198014799No Hit
GTTCAGAATAGAGCGGTTCCTTTTGGGAATCCAGGAGTGTGTAGTTGCAA2230.11156749833649358No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA2220.11106719565337028No Hit
GTGTTTACCTGGGTGGGATTCTTCAAGGAAGGCCATAGCCTCCAGGACAC2150.10756507687150725No Hit
GTTAAATCAGAAGCATGGCCCATTGGGGAGTCCCCCAAAGGAGTGGAAGA2150.10756507687150725No Hit
GATTTAACAGTGCATTTGGATCGTCAAGTTCTACTACTATTGATTCGCCT2130.10656447150526069No Hit
GCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC2120.1060641688221374No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2120.1060641688221374No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT2070.10356265540652095No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA2060.10306235272339766No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATA2060.10306235272339766No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC2040.10206174735715107No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT2030.10156144467402778No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG2020.1010611419909045No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT2020.1010611419909045No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGGG100.001475292245.00002245
GCTTATA150.0049639107163.333331
AACGCCC150.0049639107163.33333245
CTGATAG150.0049639107163.333332
GCAGGGT2850.0154.736859
CTAAATT1200.0153.1258
GAGCGAA3100.0150.161291
CAAGCTA1250.0147.04
AAGCTAA1250.0147.05
AAGCAGG17200.0142.441857
CGAAAGC3350.0138.955234
GCAGGGG1000.0134.759
AGCAGGT12250.0134.08
CAAAAGC15350.0130.081424
GCTAAAT1450.0126.724147
GCGAAAG3700.0125.8108143
CGCTCCC404.92009E-6122.500016
GCTCCCC404.92009E-6122.500017
GCAAAAG16900.0117.426033
AGCGAAA4100.0113.536582
Produced by FastQC (version 0.11.2)