FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_285.3520000005a53a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_285.3520000005a53a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105981
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC11741.1077457280078504No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC11221.0586803294930223No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT7460.7038997556165728No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA6760.6378501806927657No Hit
ATTCAGTACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT5970.5633085175644691No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5630.53122729545862No Hit
GTACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG3800.35855483530066706No Hit
TTTTTAAACTATTCAGTACTAATTGATGGCCATCCGAATTCTTTTGGTCG3630.3425142242477425No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA3560.3359092667553618No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC3550.3349657013993074No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCCTT3180.30005378322529513No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT3040.28684386824053365No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2910.27457751861182667No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2810.2651418650512828No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2530.23872203508175996No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG2500.23589133901359677No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC2460.23211707758937924No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2380.22456855474094414No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC2380.22456855474094414No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG2370.22362498938488973No Hit
GTTCTTAAATGCCATAAGCACAACATTCCCTTATACTGGAGATCCTCCAT2370.22362498938488973No Hit
ATGTCTGAAGTTCTTCCCTCCGTATTTATTTCATCCTAAGTGACGGGTTC2180.2056972476198564No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCAGTCTCGCACT2110.19909229012747567No Hit
AGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG2050.19343089799114935No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT1840.17361602551400723No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA1750.16512393730951774No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC1740.16418037195346336No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT1710.1613496758853002No Hit
CTTCCTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG1670.15757541446108264No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG1670.15757541446108264No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1660.15663184910502825No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC1650.15568828374897387No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA1590.15002689161264757No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1560.14719619554448438No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1540.14530906483237563No Hit
GCAATAAGTCAGTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGA1540.14530906483237563No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTGATGGCCATCC1530.14436549947632124No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1520.14342193412026683No Hit
GCCTCCAGGACACAGTCTGTTTGTGCATATCCACTTGGTTCATTATCCTC1510.14247836876421244No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTACTAATTGATGGCCATCCGAAT1500.14153480340815808No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA1480.1396476726960493No Hit
GAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT1450.13681697662788614No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1430.13492984591577736No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT1400.1320991498476142No Hit
CAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCG1350.12738132306734226No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1330.12549419235523349No Hit
GATCTTCATGATCTCAGAGAACTCTTCCTTCTTAATCCTTCCAGACTCGA1330.12549419235523349No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG1320.1245506269991791No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1320.1245506269991791No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA1300.12266349628707032No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA1300.12266349628707032No Hit
CCGTATTTATTTCATCCTAAGTGACGGGTTCTTTTCCTGTCTCCCCGATG1280.12077636557496156No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1270.11983280021890716No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1230.11605853879468962No Hit
GGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATG1220.11511497343863522No Hit
CATTAGCCTTTTCCCCTTTTGCAAGGTTACTCAGTTCATTGATGCTTAAT1200.11322784272652646No Hit
AAACTATTCAGTACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC1190.11228427737047207No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1150.10851001594625452No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1140.10756645059020013No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1140.10756645059020013No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT1130.10662288523414575No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT1130.10662288523414575No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1130.10662288523414575No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1110.10473575452203698No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC1110.10473575452203698No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT1080.1019050584538738No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1080.1019050584538738No Hit
GAATAAAAGAACTACGGAATCTGATGTCGCAGTCTCGCACTCGCGAGATA1070.1009614930978194No Hit
GCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATGTCTGAAGTTC1060.10001792774176504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTTT201.5551268E-7245.000029
CTGTTGT100.0014743163245.00002245
AAGAACG150.004960638163.33333245
AGCTATC150.004960638163.33333245
GGTAGTT301.1729353E-6163.333337
GAGCGAA2150.0153.83721
CGAAAGC2200.0144.772724
GCAGGGG800.0137.81259
AGCAGGT6650.0136.315788
GCGAAAG2450.0135.03
AAGCAGG9550.0133.403147
AGCAAAA7500.0125.766662
CAAAAGC7500.0125.766664
GCAGGTC4000.0119.43759
GCAGGGT950.0116.052649
AGCGAAA2900.0114.051722
GAGCAAA8200.0113.536581
CAAGCTA458.816827E-6108.888884
CCTCCAG801.2551027E-10107.187512
GCAGGTA4150.0103.313259
Produced by FastQC (version 0.11.2)