FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_279.3520000005a4b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_279.3520000005a4b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117223
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT6450.5502333159874768No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA6290.5365841174513535No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC4350.3710875852008565No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3320.2832208696245617No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA3090.26360014672888427No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2700.23033022529708333No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2580.22009332639499074No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2540.21668102676095988No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2380.20303182822483642No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2280.19450107913975928No Hit
GTATTGGGGAGTCCCCCAAAGGAGTGGAAGAAGGTTCCATTGGGAAAGTC2260.19279492932274384No Hit
CAAATAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGA2210.18852955478020525No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2140.18255803042065125No Hit
GTATCTAAGTCAGTATTCAATAGCCTGTATGCATCACCACAATTGGAAGG2120.1808518806036358No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC2110.17999880569512808No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA2090.17829265587811266No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT2000.1706149817015432No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG1980.16890883188452777No Hit
CTCCAACTCTATGCTGACAAAATGACTGCCGTCAGCATCCACAGCATTCT1960.16720268206751235No Hit
ACTCTATGCTGACAAAATGACTGCCGTCAGCATCCACAGCATTCTGCTGT1860.1586719329824352No Hit
ATATACAACAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1820.1552596333484043No Hit
CCCCAATACTGTCCAGGCCATTGTTCTGTCTCTCCCCTCGATTATTTCAA1760.15014118389735803No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1740.1484350340803426No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1700.14502273444631172No Hit
ACAAATAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTG1620.13819813517825No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1610.13734506026974228No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1590.13563891045272686No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1590.13563891045272686No Hit
CTTCCTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG1570.1339327606357114No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC1560.1330796857272037No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1540.13137353591018827No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA1510.12881431118466513No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC1500.1279612362761574No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1460.12454893664212655No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1460.12454893664212655No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1450.12369586173361882No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG1440.12284278682511113No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1410.12028356209958797No Hit
ATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGA1390.11857741228257254No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1390.11857741228257254No Hit
GTAGTAAACAGTATCTAAGTCAGTATTCAATAGCCTGTATGCATCACCAC1350.11516511264854166No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA1350.11516511264854166No Hit
GTAGTAAACAGTATCTGCAACACTACTGGAGCTGGAAAACCGAAGTTTCT1340.11431203774003396No Hit
GTTTACTACTGTCCAGGCCATTGTTCTGTCTCTCCCCTCGATTATTTCAA1330.11345896283152623No Hit
ACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG1310.1117528130145108No Hit
CACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA1300.11089973810600309No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1300.11089973810600309No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT1280.10919358828898765No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1260.10748743847197222No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTGTTGTATA1250.10663436356346452No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1240.10578128865495678No Hit
CAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGT1240.10578128865495678No Hit
GTTAAAGCACAGATTTGAAATAATCGAGGGGAGAGACAGAACAATGGCCT1190.10151591411241821No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1190.10151591411241821No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGT950.0154.736839
GCAGGGG700.0140.09
GCGAAAG1150.0138.478273
CGAAAGC1200.0132.708334
GCAAAAG6850.0107.299273
GAGCGAA1500.0106.1666641
CAAAAGC6950.0105.7553944
AAACTAT354.435261E-4105.06
TTTAAAC354.435261E-4105.03
AAGCAGG9200.097.201097
AGCAAAA7950.097.075472
GCAGGTA4600.093.206539
AGGGTTA407.5417594E-491.875019
CTAAATT407.5417594E-491.875018
CAAGCTA407.5417594E-491.875014
GCTAAAT407.5417594E-491.875017
AAGCTAA407.5417594E-491.875015
AGGGTGT552.3895258E-589.090916
AGCAGGG2900.088.7068948
AGCGAAA1800.088.472222
Produced by FastQC (version 0.11.2)