FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_264.3520000005a503.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_264.3520000005a503.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136669
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5420.3965785949995975No Hit
GGCTGGTCCAACCCTAAGTCCAAATTGCAGATAAATAGGCAAGTCATAGT3620.2648735265495467No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3060.22389861636508646No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2740.20048438197396629No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT2600.19024065442785124No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT2570.1880455699536837No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2560.18731387512896122No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2520.18438709583007118No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2500.18292370618062617No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2470.18072862170645868No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2260.1653630303872861No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2240.16389964073784105No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2150.15731438731533853No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2060.150729133892836No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2050.14999743906811347No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2000.14633896494450097No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA1860.1360952373983859No Hit
GAGCAAAAGCAGGTAGAGGTGGGAGCATGTGAAGGTGAGAGGTTATAGTA1820.13316845809949587No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT1790.13097337362532835No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1750.12804659432643833No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1750.12804659432643833No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG1720.1258515098522708No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1700.12438812020282582No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC1700.12438812020282582No Hit
GTATTAAGTAGATCTCAATAAGAAAGTGATTTTTAAACAAGTATTTGAAG1690.1236564253781033No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1690.1236564253781033No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT1680.12292473055338081No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA1660.1214613409039358No Hit
CCCCCAACACTGACCTCTGCGCTTATTCACTCATGCCCCTGCCTTAGGGC1600.11707117195560075No Hit
GTATAAGAGAAAACAGAAAATGTATTCAGTAATAACTAAAAACCACGTAC1530.11194930818254323No Hit
GATGCAACGATTCAAGTGACCCGCTTGTTGTTGCCGCGAATATCATTGGG1490.1090225288836532No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT1420.10390066511059567No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1410.10316897028587316No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA1410.10316897028587316No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1390.10170558063642815No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGTCG150.004962202163.33334245
GGTGATC150.004962202163.333348
GACGCTC150.004962202163.33334245
TAACCGT251.1624994E-4147.05
ATAGTCG251.1624994E-4147.03
GTAACCG251.1624994E-4147.04
AAGCAGG6050.0125.537197
GGCTGCT404.914473E-6122.500017
TAGTCGC302.4027174E-4122.500014
AGTCGCA302.4027174E-4122.500015
TCTATGC950.0116.052633
GGTAGTT354.4368545E-4105.07
AGCAGGG2750.0102.4545448
AGCAGGT2300.0101.195658
CGAAAGC1500.098.000014
GCAGGGT759.860742E-998.000019
AGCAAAA6550.095.3816762
GTAGAAA1300.094.230771
GCAAAAG6600.092.803033
CAGGGGT407.544463E-491.875014
Produced by FastQC (version 0.11.2)