FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_255.3520000005a461.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_255.3520000005a461.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157608
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA8300.5266230140602No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT6720.42637429572102936No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG4170.26458047814831737No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT3440.21826303233338412No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC3240.2055733211512106No Hit
GTTCCCTGTCCAAGGGCAAATTGATAACACTTGTCAGGATCGCATGACAC3160.2004974366783412No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT3050.19351809552814578No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT2960.1878077254961677No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT2780.17638698543221157No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2730.17321455763666818No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2690.1706766154002335No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2680.17004212984112482No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA2560.16242830313182072No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2250.14275925079945181No Hit
GGGTTCAACCGCTGATTTGCTCTGTTGACAAAATTCAGGTCACCTCTAAC2100.13324196741282168No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2050.1300695396172783No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2030.12880056849906096No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2000.12689711182173494No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCGTGGAACGTTATCTCCCTCTT1970.12499365514440891No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1940.1230901984670829No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1910.12118674178975687No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1860.1180143139942135No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1860.1180143139942135No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG1860.1180143139942135No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA1850.1173798284351048No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT1840.11674534287599615No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1820.11547637175777878No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1810.11484188619867013No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1800.11420740063956143No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT1760.11166945840312675No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA1730.10976600172580071No Hit
CTCCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGG1690.10722805948936602No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1640.10405563169382265No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1630.10342114613471397No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1580.1002487183391706No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT1580.1002487183391706No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC100.0014749969244.99998245
ACGATAA150.00496292163.33334245
GCAGGGT2150.0142.441859
GTATTAA750.0130.666671
TAGGCAT302.4034102E-4122.500019
CGAAAGC1450.0118.275864
GCAAAAG7500.0117.63
AGCAGGT5100.0115.294118
AAGCAGG9600.0109.739597
AGCAAAA8050.0109.565222
GCAGGTA4700.0104.2553259
GCAGGGG602.998604E-7102.083349
CAAAAGC9050.098.812154
TAGTTTT654.8303446E-794.230779
GGTAGTT654.8303446E-794.230777
AGCTTAT407.546629E-491.874991
GCTTATA407.546629E-491.874992
AGCAGGG4400.083.522728
GAGCAAA10650.082.81691
ACAAGGT903.4993718E-881.666673
Produced by FastQC (version 0.11.2)