FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_250.3520000005a2a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_250.3520000005a2a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38096
Sequences flagged as poor quality0
Sequence length251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2630.6903611927761445No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1680.4409911801763965No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC1490.3911171776564469No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1310.34386812263754724No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT1260.3307433851322974No Hit
CCTATATCCTAAGTGACGGGTTCTTTTCCTGTCTCCCCGATGTGTACTTC1120.2939941201175977No Hit
GGATATAGGTAAAGAAGACAGAAGATACGGACCAGCATTAAGCATCAATG860.2257454850902982No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG830.21787064258714825No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGAAATCTAATGTCGCA830.21787064258714825No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA780.20474590508189838No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT770.20212095758084836No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT760.19949601007979842No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGA600.15749685006299874No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC590.15487190256194877No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC580.1522469550608988No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG550.14437211255774884No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC540.14174716505669888No Hit
AGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG540.14174716505669888No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG530.1391222175556489No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT530.1391222175556489No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA520.1364972700545989No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA520.1364972700545989No Hit
CCTATAATGAGGAACCCTCTCATTTCATCCTAAGTGACGGGTTCTTTTCC490.12862242755144898No Hit
GGATGAAATGAGAGGGTTCCTCATTATAGGTAAAGAAGACAGAAGATACG480.125997480050399No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG450.11812263754724907No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT450.11812263754724907No Hit
GAATACTGACTTAGCCAATAGAGTCCTACAGACTTTCCCAATGGAACCTT430.1128727425451491No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG430.1128727425451491No Hit
CCTTTGGGGGACTCCCCAATGGGCCATGCTTCTGATTTATTCTCAAAAAA430.1128727425451491No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC410.10762284754304914No Hit
GGATGAAATGGATGATGGCAATGAAATATCCAATCACTGCTGACAAAAGG400.10499790004199916No Hit
AGCCAATAGAGTCCTACAGACTTTCCCAATGGAACCTTCTTCCACTCCTT400.10499790004199916No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT400.10499790004199916No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA400.10499790004199916No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT390.10237295254094919No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA390.10237295254094919No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTGT301.1631728E-6163.333336
GCAGGGG150.0049482174163.333339
GAAGGTC251.1559634E-4146.999986
AAACTAT601.2732926E-11142.916676
TTTAAAC352.5055979E-6140.03
CAAGGGT352.5055979E-6140.04
GCAGGGT352.5055979E-6140.09
AAGGGTG352.5055979E-6140.05
ACAAGGG352.5055979E-6140.03
CGAAAGC652.3646862E-11131.923084
AACTATT652.3646862E-11131.923087
AGCAGGG750.0130.666678
GCGAAAG704.1836756E-11122.53
GGTCCGG302.389238E-4122.59
AAGCAGG2400.0122.57
GAGCAAA1700.0115.294111
CAAAAGC1900.0109.605264
TTTTAAA458.743811E-6108.888882
AGGTCCG354.4120167E-4105.08
AGCAGGT1650.0103.939398
Produced by FastQC (version 0.11.2)