FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_247.3520000005a220.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_247.3520000005a220.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130640
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT20811.5929271279853032No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA10530.806031843233313No Hit
GCCATGGAACAGGGACAGGGTACACTATGGACACAGTCAACAGAACACAC8150.6238518064911206No Hit
GTGTTTACCTGGGTGGGATTCTTCAAGGAAGGCCATAGCCTCCAGGACAC8000.612369871402327No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA5570.4263625229638702No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC5560.4255970606246173No Hit
GCCATGGACACAGTCAACAGAACACACCAATATTCAGAGAGGGGGAAGTG5440.41641151255358233No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG5320.40722596448254744No Hit
GTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAATGCATGTGTC4900.3750765462339253No Hit
GCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTC4560.3490508266993264No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAACCC4330.3314451928965095No Hit
GAGCGAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT4290.3283833435394979No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3700.28322106552357623No Hit
ATTCTGCACAAACAGACTGTGTCCTGGAGGCTATGGCCTTCCTTGAAGAA3570.27327005511328845No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3320.2541334966319657No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2950.2258113900796081No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2900.22198407838334355No Hit
GCCCAATTAGCACATTAGCCTTTTCCCCTTTTGCAAGGTTACTCAGTTCA2890.22121861604409063No Hit
TATCCACTTGGTTCATTATCCTCAGGTAGTGGTCCATCAATTGGGTTGAG2810.2150949173300674No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTGT2770.2120330679730557No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2720.20820575627679117No Hit
GTCCAGGCCATTGTTCTGTCTCTCCCTTCGATTATTTATTGATTCGCCTT2710.20744029393753827No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTAAAAG2590.19825474586650338No Hit
GCCATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2570.19672382118799756No Hit
AGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTT2480.1898346601347214No Hit
CTGTCCAGGCCATTGTTCTGTCTCTCCCTTCGATTATTTATTGATTCGCC2460.18830373545621557No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2430.18600734843845684No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2400.1837109614206981No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2390.1829454990814452No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA2360.18064911206368645No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA2350.17988364972443355No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2340.17911818738518065No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2330.17835272504592775No Hit
TAGTAGCACTGCCATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA2160.1653398652786283No Hit
GTATACTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC2100.16074709124311085No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2100.16074709124311085No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2070.15845070422535212No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2050.1569197795468463No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA2030.15538885486834048No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1990.15232700551132886No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1950.1492651561543172No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGGTTGTCCCT1940.1484996938150643No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1920.14696876913655849No Hit
CACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGATCA1910.1462033067973056No Hit
AGCGAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTT1880.14390691977954684No Hit
GTCCCTAAGAGCCTGAACAACAAGGAGCAGTTTTCTTGACTCCGCTGAAA1870.14314145744029394No Hit
ATGATCACCACAATTGGAAGGATTTTCAGCGGAGTCAAGAAAACTGCTCC1850.14161053276178812No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1820.1393141457440294No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1760.13472137170851195No Hit
CCTCCAAGTGAGTGCATATTGCTGCAAATTTGTTGGTTTCAATTTTCAGA1720.1316595223515003No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1720.1316595223515003No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCT1700.13012859767299448No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1690.12936313533374158No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAAACGCATTTGAAAAAAAGACG1670.12783221065523576No Hit
GTTTACCTGGGTGGGATTCTTCAAGGAAGGCCATAGCCTCCAGGACACAG1650.12630128597672993No Hit
GTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATGCA1650.12630128597672993No Hit
GATCTTCATGATCTCAGAAAACTCTTCCTTCTTAATCCTTCCAGACTCGA1620.12400489895897121No Hit
CTATTGGCTAAGTCAGTATTCAATAGCCTGTATGCATCACCACAATTGGA1600.1224739742804654No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC1510.1155848132271892No Hit
GATGCATACAGGCTATTGAATACTGACTTAGCCAATAGAGTCCTACAGAC1510.1155848132271892No Hit
CTTAGGGACAACCTCGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA1490.1140538885486834No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA1490.1140538885486834No Hit
GCCCAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACC1480.1132884262094305No Hit
ATTCTAAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCA1450.11099203919167178No Hit
GCTCTATTCTGCACAAACAGACTGTGTCCTGGAGGCTATGGCCTTCCTTG1450.11099203919167178No Hit
GAACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTAGAA1430.10946111451316597No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT1420.10869565217391304No Hit
ACAAATAGTAGCACTGCCATAACTATTTTAATGCATGTGTCAGGAAGGAG1410.10793018983466013No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1390.10639926515615432No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG1370.10486834047764851No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT1360.10410287813839558No Hit
TCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1360.10410287813839558No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAT100.0014747082245.00002245
GATTACG100.0014747082245.00002245
AGGTCTT100.0014747082245.00002245
GAGCGAA1600.0168.43751
GCAGGGT1900.0167.631589
CGTTCTT150.0049619526163.33333245
GCAGGGG1050.0163.333339
GCTAAAT600.0163.333337
AAGCAGG12400.0161.028217
AGCAGGG3200.0153.1258
CTAAATT650.0150.769238
AGCAGGT8850.0146.723188
GCAAAAG11850.0145.759493
CAAAAGC12000.0143.93754
CAAGCTA700.0140.04
AAGCTAA700.0140.05
CGAAAGC1950.0138.205124
GCAGGTA7650.0136.111119
AGCAAAA13150.0133.212922
TAAATTC851.8189894E-12115.294119
Produced by FastQC (version 0.11.2)