FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_238.3520000005a257.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_238.3520000005a257.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200487
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG3610.18006155012544456No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA3570.17806640829579973No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGAATGATGATGGGCAT3280.16360163003087483No Hit
GTTTCAAAATATACCACAAATGTGACAATGCCTGCATAGGATCAATAAGA2980.1486380663085387No Hit
GATGCAACGATTCAAGTGACCCGCTTGTTGTTGCCGCGAATATCATTGGG2870.14315142627701546No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2790.13916114261772586No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2710.1351708589584362No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG2620.13068178984173537No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA2520.12569393526762335No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT2490.12419757889538972No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2380.11871093886386648No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2360.11771336794904408No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2330.11621701157681047No Hit
TCCCTGAAGTCTGCTTAAAGTGGGAGCTGATGGATGAGAATTATCGGGGA2310.11521944066198805No Hit
TCATAAGGGTAACAGTTGCTGTAGGCTTTGCTTCGTTCAACAAAAAGGTC2190.10923401517305362No Hit
TCCCTGTACACATTGTGGTCATAAGTTCCATTTCTTATTGATCCTATGCA2190.10923401517305362No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA2190.10923401517305362No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2170.10823644425823122No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA2160.10773765880082No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2100.10474494605635279No Hit
GTATGTAATCATCGCCAAAAACATTCGGGGGTTTTGATTTTCATTCCACT2050.10225101876929676No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2020.10075466239706314No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA2010.10025587693965196No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT2010.10025587693965196No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATATT600.0163.333332
ATATTCA700.0140.04
GCAGGGG302.4043766E-4122.58
TATATTC851.8189894E-12115.294113
TCTATGC1200.0112.2916643
TCCAACT901.8189894E-12108.888892
GGCTGGT501.491345E-598.01
CGAAAGC654.835165E-794.230773
GCAGGGT407.5496477E-491.875019
GTCCAAC552.3936891E-589.090916
AGGGTGT707.5163007E-787.56
CAACTCT1152.3646862E-1185.217394
TACGGAT450.001205373881.666672
TACTGAT450.001205373881.666677
CGAATAA450.001205373881.66667245
CAACCCT603.685843E-581.6666649
CCAACTC1254.9112714E-1178.3999943
CCAACCC655.4811768E-575.384618
ACGGATA500.001831204573.53
CTAGAGT1401.3460522E-1070.06
Produced by FastQC (version 0.11.2)