FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_237.3520000005a1c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_237.3520000005a1c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97066
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG6070.6253477015638844No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC5180.5336575113840067No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5050.5202645622566089No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC3140.32349123276945585No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT2750.2833123853872623No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT2700.27816125110749385No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2660.27404034368367913No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2530.2606473945562813No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT2520.2596171677003276No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT2500.2575567139884203No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT2330.24004285743720769No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA2270.2338614963014856No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2060.21222673232645828No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1920.19780355634310673No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT1670.1720478849442647No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA1600.16483629695258897No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC1560.16071538952877423No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1500.15453402839305216No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC1490.15350380153709847No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA1470.15144334782519112No Hit
GGTTTCATACCCTAAGCGTGACGTCTCGTTGATTGTTCTCCCCATCCACA1450.14938289411328376No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG1440.14835266725733007No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA1430.14732244040137638No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG1400.14423175983351533No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC1360.14011085240970061No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC1360.14011085240970061No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT1300.13392949127397852No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT1260.1298085838501638No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT1220.1256876764263491No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1220.1256876764263491No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT1200.12362722271444172No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG1180.12156676900253437No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1170.12053654214658069No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCGTGGAACGTTATCTCCCTCTT1170.12053654214658069No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1160.119506315290627No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG1160.119506315290627No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT1110.11435518101085859No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1110.11435518101085859No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGT1090.11229472729895124No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1050.10817381987513651No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1050.10817381987513651No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT1030.10611336616322914No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1020.10508313930727546No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG1020.10508313930727546No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT1000.10302268559536809No Hit
GGTCCCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTT990.10199245873941441No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA990.10199245873941441No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC980.10096223188346073No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGCGATGGCTTTCAAAATAA980.10096223188346073No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAG151.5151003E-5245.05
GAGTGGG150.0049599996163.33333245
CCATGGG150.0049599996163.33333245
AGTCTAA150.0049599996163.33333245
GACGGCC150.0049599996163.33333245
GCAGGGT1400.0157.59
TTTACAC251.1614686E-4147.03
AAGCAGG5250.0142.333337
AGCAGGT2950.0137.03398
GCAGGGG455.3772965E-8136.111119
AGCAAAA4500.0130.666672
CTCCGTC302.4005923E-4122.5245
GCAAAAG4950.0121.2626343
CGAAAGC1350.0117.962974
GAGCAAA5200.0113.076921
TACGGAT458.813062E-6108.888892
AGCAGGA458.813062E-6108.888898
GCGAAAG1500.0106.1666643
TTTAAAC501.4874491E-598.03
CGGATAA501.4874491E-598.04
Produced by FastQC (version 0.11.2)