FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_235.3520000005a4a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_235.3520000005a4a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134665
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA14251.0581814131363012No Hit
TAATCAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGC10490.7789700367578807No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA8780.6519882671815245No Hit
GCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC4290.31856829911261275No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4070.3022314632606839No Hit
AAACAAGGGTGTTTTTAATCAATGCACTCAAATGCAAATGTTGCACCTAA3770.2799539598262355No Hit
GTTTTTAATCAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT3630.2695577915568262No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG3510.2606467901830468No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3500.2599042067352319No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3240.24059703709204322No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3080.22871570192700405No Hit
GAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA3060.22723053503137416No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2970.22054728400103962No Hit
AGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT2970.22054728400103962No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2960.21980470055322468No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2900.215349199866335No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT2770.20569561504474065No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2700.20049753091003603No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2590.19232911298407157No Hit
AGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT2560.19010136264062674No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2530.18787361229718189No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2520.18713102884936694No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2520.18713102884936694No Hit
GTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGC2480.18416069505810714No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2360.17524969368432777No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2320.17227935989306797No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2290.17005160954962315No Hit
GTAGAAACAAGGGTGTTTTTAATCAATGCACTCAAATGCAAATGTTGCAC2290.17005160954962315No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2240.1663386923105484No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2110.15668510748895406No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG2080.15445735714550923No Hit
CTTCCTTCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG2040.15148702335424943No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1990.1477741061151747No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1940.14406118887609995No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1800.1336650206066907No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT1770.13143727026324584No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1770.13143727026324584No Hit
TTTTTAATCAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTT1750.12995210336761595No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT1730.12846693647198604No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT1640.12178368544165151No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1610.11955593509820667No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC1570.11658560130694687No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC1570.11658560130694687No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC1550.11510043441131698No Hit
AATCAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCA1530.11361526751568708No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA1500.11138751717224224No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC1490.11064493372442728No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCGTGGAACGTTATCTCCCTCTT1430.1061894330375376No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT1430.1061894330375376No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA1400.10396168269409276No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC1390.10321909924627781No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT1360.10099134890283297No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGAAGC100.0014747591244.99998245
TGGTTAA150.004962122163.33334245
GCTCAGT150.004962122163.333343
GCAAAAG7650.0132.90853
GAGCGAA1300.0131.923081
GCAGGGT5450.0128.119269
CGAAAGC2000.0116.3753
CAAAAGC8750.0116.2000054
AAGCAGG11400.0105.3070147
AGCGAAA2250.0103.444451
AGCAGGG6900.0102.971018
GAGCAAA10250.098.01
CCAGACC407.544222E-491.87499245
GCGAAAG2600.089.519232
AAAAGCA11650.088.326185
ACAAGGT707.502822E-787.58
TAGAAAC2400.086.770842
AGCAAAA11950.085.083682
AGCAGGT4500.084.388898
GATGGCC450.001204509281.666674
Produced by FastQC (version 0.11.2)