FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_220.3520000005a42e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_220.3520000005a42e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183102
Sequences flagged as poor quality0
Sequence length251
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA7380.4030540354556477No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2840.15510480497209206No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2820.15401251761313364No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2800.15292023025417525No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT2650.14472807506198732No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2600.14199735666459132No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2340.1277976209981322No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA2310.1261591899596946No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2310.1261591899596946No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA2260.1234284715622986No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2250.1228823278828194No Hit
TCCTACAGCACCCTGGAATGGCTGAAATTAAGTATTTTATATGGCAAAAT2220.12124389684438182No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2170.11851317844698583No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2140.11687474740854824No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2140.11687474740854824No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT2130.11632860372906904No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2010.10977487957531869No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2000.10922873589583948No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1980.10813644853688109No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA1900.1037672991010475No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAA150.004963573163.333331
AGCAAAA9950.0137.889452
GCAAAAG9850.0136.802033
GAGCAAA11650.0120.9227451
CAAAAGC11300.0119.2477954
GAGCGAA1650.0118.787881
GCGAAAG2300.0117.173912
CGAAAGC2350.0114.6808553
AAGCAGG15550.0100.048237
AAAAGCA13900.096.9424445
AGCGAAA2800.096.251
AAAGCAG17700.087.2033846
AGCAGGG7400.082.770278
AGCAGGA3850.076.363648
ATATCGT954.6135083E-664.473691
GCAGGGT6100.064.26239
TATATCG600.003772009261.253
TATCGTG1006.254244E-661.252
GCAGGTA4600.058.5869569
AGCAGGT8850.058.135598
Produced by FastQC (version 0.11.2)