FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_203.3520000005a2b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_203.3520000005a2b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145556
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT11290.7756464865756135No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA10220.7021352606556927No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG8190.56267003764874No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC6950.4774794580779906No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT4660.3201516941933002No Hit
TAATTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAAT4660.3201516941933002No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT4420.3036631949215422No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT4420.3036631949215422No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4100.28167852922586495No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA3600.24732748907636926No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC3460.23770919783451042No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC3350.23015196900162135No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3340.22946494819863147No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA3250.22328176097172223No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3160.21709857374481298No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA3110.21366346972986344No Hit
GTTAAAGCACAGATTTGAAATAATCGAGGGGAGAGACAGAACAATGGCCT2950.2026711368820248No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC2940.20198411607903485No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2770.19030476242820632No Hit
CTATTAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC2670.18343455439830716No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC2650.18206051279232735No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA2530.17381626315644838No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2520.17312924235345847No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2520.17312924235345847No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2430.16694605512654923No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2340.16076286789964No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2310.15870180549067026No Hit
CTTCCTTCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG2300.15801478468768032No Hit
GTGTAATTATGGCCATATGGTCCACTGTGGTTTTTGTCAGTATCTCGCGA2280.15664074308170053No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2140.1470224518398417No Hit
GGAGCAGTTTTCTTGACTCTGCTGAAAATCCTTCCAATTGTGGTGATGCA2110.14496138943087197No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2110.14496138943087197No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT2080.14290032702190222No Hit
GTGTCAACAGTGCATTTGGATCGTCAAGTTCTACTACTATTGATTCGCCT2080.14290032702190222No Hit
GTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA2040.14015224380994257No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA2000.1374041605979829No Hit
GAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT1990.13671713979499298No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC1990.13671713979499298No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1970.13534309818901316No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1970.13534309818901316No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1860.1277858693561241No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT1850.1270988485531342No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1830.12572480694715435No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1830.12572480694715435No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1800.12366374453818463No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1770.12160268212921486No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1740.11954161972024513No Hit
GCAATGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATGCA1740.11954161972024513No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1690.11610651570529555No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG1680.11541949490230564No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1650.11335843249333591No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1620.11129737008436615No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1610.11061034928137624No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA1600.10992332847838632No Hit
ATAAATGGTCACACAAAGAACAATAGGGAAGAGCCTGTATGCATCACCAC1530.10511418285745693No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1530.10511418285745693No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1520.10442716205446702No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1480.10167907884250735No Hit
AGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT1470.10099205803951743No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTGG150.0049625323163.333342
AAGGTAC301.1744287E-6163.333349
CACGAGT150.0049625323163.33334245
AGACGGG150.0049625323163.33334245
GCAGGGT1750.0161.09
CGAAAGC2400.0142.916674
GCAGGGG850.0129.705899
AAGCAGG11600.0123.556047
TCGTACT501.010867E-7122.52
GCGAAAG2900.0118.275863
AGCAAAA9950.0112.035182
GTCGTAC551.7845741E-7111.363641
CGTACTC551.7845741E-7111.363643
AGCAGGG3650.0110.7534268
GAGCGAA3150.0108.888891
GCAAAAG10050.0108.482593
CAAAAGC10250.0106.365854
TAGATGG354.4374412E-4105.08
AGCAGGT7650.0104.084978
GCAGGTC3100.0102.741939
Produced by FastQC (version 0.11.2)