FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_189.3520000005a1eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_189.3520000005a1eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140904
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTAACCGTACCAACCATCCACCATTCCTTCCCAACCATTTTCTATGA9130.647958893998751No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC8440.5989893828422188No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA6090.43220916368591383No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT5130.3640776699029126No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA4960.3520127178788395No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC4460.31652756486685973No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA4320.3065917220235054No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT4120.29239766081871343No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT4110.29168795775847384No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT3350.23775052518026457No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3290.23349230681882702No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC3230.2292340884573894No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA3190.22639527621643105No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2580.1831033895418157No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2510.17813546812013853No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2490.17671606199965934No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2450.17387724975870095No Hit
CCAAGAGCCTGAACAATAAGGAGCAGTTTTCTTGACTCTGCTGAAAATCC2420.17174814057798218No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA2390.1696190313972634No Hit
GCCCCCCAAGATCAAAGGTCCCAGGTTCGAGCTTGTCCCTAAGAGCCTGA2320.1646511099755862No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA2300.16323170385510702No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2260.16039289161414863No Hit
GTCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGTCAAGAAAA2200.15613467325271108No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2090.1483279395900755No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2040.14477942428887752No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2020.14336001816839833No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2000.14194061204791916No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1970.13981150286720037No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA1960.13910179980696077No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1920.13626298756600239No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1870.1327144722648044No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1850.1312950661443252No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1840.1305853630840856No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1790.12703684778288762No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG1790.12703684778288762No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT1730.12277862942145007No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1720.12206892636121046No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATAGTAGCAC1720.12206892636121046No Hit
GTCCCTAAGAGCCTGAACAATAAGGAGCAGTTTTCTTGACTCTGCTGAAA1700.12064952024073128No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT1630.11568159881905411No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT1620.11497189575881452No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT1560.11071367739737693No Hit
GTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGTCAAGAAAACTGC1530.10858456821665814No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG1520.10787486515641855No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1510.10716516209617896No Hit
ATTGTGGATTCTTGATCGTCTTTTTTTCAAATGCGTCTATCGACTCTTCA1490.10574575597569977No Hit
ACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG1460.10361664679498098No Hit
GGATTATACCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGT1420.1007778345540226No Hit
AGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT1420.1007778345540226No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGT1100.0245.000035
GTAACCG1100.0245.000034
CGGGAGG100.0014748315244.99998245
CAGTACG1650.0148.484859
CCTGTAA2050.0131.463411
GGACAGT2350.0109.468096
GCAGGGG458.827006E-6108.888899
ACAGTAC2300.0106.5217368
AACCGTA2650.0106.3207556
AGGTCGT354.437144E-4105.06
AGCAGGT3200.0103.359378
CGAAAGC1200.0102.0833363
ACTATTC1100.0100.227288
TTTAAAC1100.0100.227283
TCCAACT1100.0100.227282
CTATTCA1150.095.869569
CGTACCA2950.095.508479
GACAGTA2750.093.5454567
TGGACAG2800.091.8755
AAGCAGG4800.091.8757
Produced by FastQC (version 0.11.2)