FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_182.3520000005a3cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_182.3520000005a3cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169226
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA8540.5046505856074125No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT4770.28187158001725504No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3450.20386938177348635No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2980.17609587179275052No Hit
CTTCCTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG2780.16427735690733103No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2360.13945847564795008No Hit
TTCCTATACAGTTTAACTGCTTTATCCATGTTATTTGGGTCTCCATTCCC2250.13295829246096935No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT2230.13177644097242738No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2210.13059458948388544No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2150.1270490350182596No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT2120.12527625778544668No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2090.12350348055263376No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1970.11641237162138207No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1930.11404866864429815No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG1910.1128668171557562No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC1860.10991218843440134No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT1820.10754848545731745No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1820.10754848545731745No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGAGAACTCGTCCTTTATGA1810.10695755971304646No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1740.10282107950314963No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1730.10223015375887866No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT1730.10223015375887866No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1710.10104830227033672No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGCTA452.8194336E-10163.333344
CTAGAGC150.004963241163.333334
TCTTACC150.004963241163.33333245
AGGTCGT150.004963241163.333336
GCTAAAT505.875336E-10147.07
AAGCTAA505.875336E-10147.05
GCAGGGT1200.0132.708339
CGAAAGC1200.0122.54
CTAAATT602.0936568E-9122.58
GCAGGGG1000.0122.59
GCAAAAG5800.097.1551743
AGCAGGT3250.094.2307748
AAGCAGG7750.090.096777
AGCAAAA6450.085.465112
TCGCTCT450.00120504781.66667245
ACAAGGT751.1323336E-681.6666643
GCGAAAG2000.079.6253
CAAAAGC7200.078.263894
GAGCGAA1950.075.384621
TATATTC655.478705E-575.384613
Produced by FastQC (version 0.11.2)