FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_171.3520000005a121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_171.3520000005a121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156958
Sequences flagged as poor quality0
Sequence length251
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA13210.8416264223550249No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT9880.629467755705348No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT7260.46254412008307955No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG5610.3574204564278342No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG4930.31409676473961184No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC4350.27714420418201047No Hit
TTTTTGGACAGTACGGATAACAAATAGAAGCACTGCTATAACTATTTTAA4260.2714101861644516No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4100.26121637635545814No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3850.24528854852890583No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3390.21598134532804955No Hit
GTACGGATAACAAATAGAAGCACTGCTATAACTATTTTAATGCATGTGTC3190.20323908306680766No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC3160.20132774372762138No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2940.18731125524025535No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2720.17329476675288932No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT2720.17329476675288932No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCTACAGCATTCTGCTGT2700.17202054052676513No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2670.17010920118757886No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2640.16819786184839258No Hit
GCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTTGA2540.16182673071777162No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC2540.16182673071777162No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA2450.1560927127002128No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2440.1554555995871507No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2430.1548184864740886No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCTACAGCATTCT2400.15290714713490233No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT2330.14844735534346767No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT2330.14844735534346767No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2290.14589890289121932No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC2160.1376164324214121No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2100.13379375374303953No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2040.12997107506466699No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2030.12933396195160488No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT2010.12805973572548068No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT2010.12805973572548068No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2010.12805973572548068No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1900.12105149148179768No Hit
GAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT1840.11722881280342512No Hit
CTATTAATGTGCAGTTTTCTCCATCAAGGATCTGATGAGGACTGTCGCAT1830.11659169969036304No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1820.11595458657730094No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT1780.11340613412505257No Hit
GCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGGCG1770.11276902101199048No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG1670.10639788988136953No Hit
TTCCATGAGAGCCTCAAGATCTGTGTTTTTCCCAGCAAAGACATCTTCAA1670.10639788988136953No Hit
AGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAA1670.10639788988136953No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT1640.10448655054218325No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1630.10384943742912116No Hit
GTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGTGTT1620.10321232431605906No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT1580.10066387186381069No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1580.10066387186381069No Hit
TCATAAGGGTAACAGTTGCTGTAGGCTTTGCTTCGTTCAACAAAAAGGTC1570.1000267587507486No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT1570.1000267587507486No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCTAA254.7369758E-7196.05
CTAAATT301.1747179E-6163.333338
GCAGGGT2200.0128.068189
GCAAAAG9350.0123.155083
GCTAAAT404.9167684E-6122.500017
GCAGGGG1550.0118.548399
AGCAAAA10950.0109.634712
CAAGCTA458.830164E-6108.888894
ACAAGGT901.8189894E-12108.888898
AAGCAGG12100.0108.3264547
AGCAGGT7000.0103.250018
GCAGGTA6950.0100.467619
AGCAGGG4650.0100.107538
CGAAAGC1600.099.531264
CAAAAGC11750.098.000014
TAAGAGT501.4903311E-598.04
GAGCAAA12400.095.826621
CAAGGGT1200.091.8758
CGGATAA1500.089.833334
ACGGATA1500.089.833333
Produced by FastQC (version 0.11.2)