FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_168.3520000005a29a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_168.3520000005a29a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170639
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA17221.009147967346269No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG10080.5907207613734258No Hit
ATTAAAGTGCAGTTTTTTCCATCAAGGATCTGATGAGGACTGTCGCATAT7440.436008181013719No Hit
GAGCAAAAGCAGGGGATAATTCTATCAACCATGAAGACTATCATTGCTTT7080.4149110109646681No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG6360.3727166708665663No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT5290.310011193220776No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA5140.30122070570033815No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC4570.26781685312267417No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC4360.25551017059406117No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG4270.2502358780817984No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT4010.23499903304637276No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3970.23265490304092265No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT3900.22855267553138497No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3830.2244504480218473No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC3810.22327838301912226No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG3580.20979963548778416No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3450.20218121297007133No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG3240.18987453044145827No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA3180.18635833543328315No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC3150.18460023792919555No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC3120.182842140425108No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA3020.17698181541148272No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2980.1746376854060326No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2920.17112149039785746No Hit
TGAAAACTATCATTGCTTTGAGCTACATTCTATGTCTGGTTTTCGCTCAA2850.16701926288831978No Hit
GTATATGGGTCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGTGTT2700.15822877536788188No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2610.15295448285561916No Hit
CCTGTATATGGGTCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT2530.14826622284471896No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA2520.14768019034335644No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA2510.14709415784199392No Hit
ATATATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC2490.14592209283926888No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT2440.14299193033245622No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT2400.14064780032700613No Hit
GAATCGTTGCATCTGCACCCCCATTCGTTTCTGATAGGTCTGCAAATTTT2380.1394757353242811No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT2320.13595954031610594No Hit
ATATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGCTA2310.13537350781474342No Hit
GTTATATATCGCGAAGCAGGTGTTGAGACCATGGTCATTTTAAGTGCCTC2260.13244334530793078No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT2220.13009921530248067No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2210.12951318280111815No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA2200.12892715029975563No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATGGACGCATTTGAAAAAAAGACG2170.12716905279566806No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC2140.12541095529158047No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA2130.12482492279021794No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG2100.12306682528613036No Hit
TTCCATGAGAGCCTCAAGATCTGTGTTTTTCCCAGCAAAGACATCTTCAA2060.12072269528068028No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC2050.12013666277931774No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC2050.12013666277931774No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT2010.11779253277386763No Hit
CACTCTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAA2000.11720650027250512No Hit
ATCCCAATGATATTCGCGGCAACAACAAGCGGGTCACTTGAATCGTTGCA1990.1166204677711426No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1990.1166204677711426No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC1970.11544840276841754No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT1970.11544840276841754No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA1920.11251824026160492No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA1870.10958807775479228No Hit
CCCATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTA1860.10900204525342976No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGGCTTGAA1850.10841601275206722No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCGTGGAACGTTATCTCCCTCTT1840.1078299802507047No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT1840.1078299802507047No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGT1810.10607188274661714No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC1780.10431378524252954No Hit
GCAAAAGCAGGGGATAATTCTATCAACCATGAAGACTATCATTGCTTTGA1760.10314172023980449No Hit
TCATAAGGGTAACAGTTGCTGTAGGCTTTGCTTCGTTCAACAAAAAGGTC1760.10314172023980449No Hit
AGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAA1730.10138362273571692No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGG1710.10021155773299187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGG1550.0158.064519
GCAGGGT3300.0155.909099
AGCAAAA11250.0138.28892
CAAAAGC11900.0129.705894
GCAAAAG12000.0128.6253
AAGCAGG14600.0124.1780857
AGCAGGG6150.0123.495948
CGAAAGC2400.0117.3958364
GCAGGTA6800.0115.294139
GAGCAAA14200.0109.559851
AGCAGGT8050.0108.043498
GAATACG354.4387652E-4105.0245
TGTAGCC354.4387652E-4105.04
GCGAAAG2900.097.155173
AAAAGCA16900.091.331365
AGGGTGT552.3926636E-589.090916
CAACTCT1101.6370905E-1189.090914
ATTAAAG2300.085.217391
AAAGCAG21900.082.785396
AACTATC1051.0877557E-981.6666645
Produced by FastQC (version 0.11.2)