FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_162.3520000005a213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_162.3520000005a213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135269
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT24971.8459514005426225No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA12390.9159526572976809No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA10670.7887986160909003No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT8660.6402058121225115No Hit
GATCTTCATGATCTCAGAGAACTCTTCCTTCTTTATCCTTCCAGACTCGA7810.5773680592005559No Hit
CTTCCTTCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG7790.5758895238376864No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA6860.5071376294642527No Hit
CTATAGGATGAACAGATGTACCAGAAGTGCTGCAACTTGTTCGAGAAATT6590.48717740206551385No Hit
GTTGACTGTGTCCATAGTGTACCCTGTCCCTGTTCCATGGCTGTATGGAG5410.39994381565621095No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT4470.3304526536013425No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTT3770.2787039159009086No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA3770.2787039159009086No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3680.2720505067679956No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3630.26835416836082177No Hit
GTTCTTAAAAGTTCCAGCGCAAAATGCCATAAGCACAACATTCCCTTATA3530.2609614915464741No Hit
CTCTTCCTTCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCC3500.2587436885021698No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG3390.2506117440063873No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3310.2446976025549091No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA3290.24321906719203956No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3220.23804419342199618No Hit
TCCTCAAGAATTCCCCTTTGGCTTGTGTTTAGAATAGAGCGGTTCCTTTT3120.23065151660764843No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGTTC2770.2047771477574315No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2650.19590593558021424No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA2620.19368813253590994No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2550.18851325876586653No Hit
CTATGGACACAGTCAACAGAACACACCAATACTCAGAGAGGGGGAAGTGG2530.18703472340299698No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2440.18038131427008405No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2440.18038131427008405No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2390.1766849758629102No Hit
TCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGGGCCCTA2340.17298863745573637No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCT2300.17003156672999725No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2270.16781376368569295No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2220.1641174252785191No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC2190.16189962223421478No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2110.15598548078273664No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2110.15598548078273664No Hit
ACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCTGGTACATCTGTT2090.1545069454198671No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC2030.15007133933125846No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2020.14933207164982368No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2000.14785353628695416No Hit
GCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC1980.1463750009240846No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGTT1970.14563573324264983No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1960.14489646556121505No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCCTTCTTT1960.14489646556121505No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC1930.14267866251691075No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1890.13972159179117166No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1880.1389823241097369No Hit
GGCCTCCACCATGCTAGAAATTCCAATCGGTCTCCTATATGAACTACTAG1850.13676452106543258No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1780.1315896472953892No Hit
GATCTTATGATCTCAGAGAACTCTTCCTTCTTTATCCTTCCAGACTCGAA1750.1293718442510849No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC1720.12715404120678056No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1680.12419697048104147No Hit
GTAGTAAACAGTATCTGCAACACTACTGGAGCTGGAAAACCGAAGTTTCT1660.12271843511817195No Hit
TTCCAGACTCGAAGTCAATTCTGGCATCAATCCGGGCCCTAGACACCATG1660.12271843511817195No Hit
ATCCTATAGTGTACCCTGTCCCTGTTCCATGGCTGTATGGAGGATCTCCA1640.1212398997553024No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC1620.11976136439243285No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1620.11976136439243285No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT1560.11532575830382422No Hit
TCCTATAGTGTACCCTGTCCCTGTTCCATGGCTGTATGGAGGATCTCCAG1540.11384722294095469No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1520.11236868757808516No Hit
GAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA1510.11162941989665037No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGTT1460.10793308148947653No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1460.10793308148947653No Hit
GAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT1440.10645454612660699No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT1430.10571527844517221No Hit
AGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAA1420.10497601076373743No Hit
ACTATAGGATGAACAGATGTACCAGAAGTGCTGCAACTTGTTCGAGAAAT1390.10275820771943311No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1390.10275820771943311No Hit
CTATGGACACAGTCAACAGAACACACCAATACACACTCCTGGATTCCCAA1380.10201894003799836No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1370.10127967235656359No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAAAG11100.0168.851353
GAGAGCG150.0049621463163.33333245
CACTACG150.0049621463163.33333245
CAAAAGC12400.0153.1254
AAGCAGG14700.0150.833347
GAGCAAA13300.0143.684221
GCAGGGT2650.0138.679249
AGCAAAA14150.0132.455832
GAGCGAA2750.0129.181811
AGCAGGC7700.0127.272728
GCAGGTA5800.0122.59
AAGGGTG1700.0122.55
AGCAGGT6200.0120.5241858
AGCAGGG3800.0119.276318
CGAAAGC3000.0118.416674
AAAAGCA16200.0117.2067955
AAAGCAG19300.0116.152856
CAAGGGT1900.0116.052634
ACAAGGG1900.0116.052633
AGGGTGT1800.0115.6944356
Produced by FastQC (version 0.11.2)