FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_159.3520000005a510.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_159.3520000005a510.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109084
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA6550.6004546954640461No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT6300.5775365773165634No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA4380.40152542994389645No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT2880.26401672105900037No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA2880.26401672105900037No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2710.2484324007187122No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA2680.24568222654101426No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2590.23743170400792052No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2570.2355982545561219No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2470.2264310072971288No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2410.220930658941733No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2370.21726376003813574No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2220.20351288914964616No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2210.2025961644237468No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC2090.19159546771295516No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2050.18792856880935793No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA1950.17876132155036487No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC1920.17601114737266693No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1880.1723442484690697No Hit
CTACTGTTCTTAAAAGTTCCAGCGCAAAATGCCATAAGCACAACATTCCC1870.17142752374317038No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1850.1695940742913718No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1820.16684390011367387No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC1760.16134355175827803No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1750.1604268270323787No Hit
GATCTTCATGATCTCAGAGAACTCTTCCTTCTTTATCCTTCCAGATTCGA1740.1595101023064794No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1710.15675992812878148No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT1680.15400975395108357No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1660.15217630449928496No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC1650.15125957977338564No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT1640.15034285504748635No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA1620.14850940559568773No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1600.1466759561438891No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1590.1457592314179898No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1550.14209233251439257No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1540.14117560778849328No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT1510.13842543361079535No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG1480.13567525943309744No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1460.13384180998129883No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1400.12834146162590299No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA1350.12375783799640644No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC1340.12284111327050713No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT1330.12192438854460783No Hit
GCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC1310.12009093909280923No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1280.11734076491511129No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1280.11734076491511129No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA1250.11459059073741337No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1230.11275714128561476No Hit
GATCTTCTTCTTTATCCTTCCAGATTCGAAGTCAATTCTGGCATCAATCC1200.11000696710791683No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA1130.10358989402662168No Hit
GTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATGCA1110.10175644457482308No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1100.10083971984892377No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT1100.10083971984892377No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGG100.0014743754245.000026
GAGCGAA650.0188.461531
TAGGTAG150.0049608364163.333339
GGCTGCC150.0049608364163.33333245
TTATTAG150.0049608364163.333335
CAAAGTA150.0049608364163.33333245
GCAAAAG5350.0123.644863
GCAGGGG302.4013994E-4122.57
CAAGCTA501.00913894E-7122.499994
AAGCTAA501.00913894E-7122.499995
GCTAAAT551.7815364E-7111.363647
CAAAAGC6300.0106.9444354
AAGCAGG7850.0102.993637
CGAAAGC1350.099.814814
AGCAGGT4700.099.042558
TCCAACT759.842552E-998.02
GCAGGTA3900.097.371799
CTAAATT654.8202855E-794.230778
AGCAGGA801.540866E-891.875018
GCGAAAG1500.089.8333363
Produced by FastQC (version 0.11.2)