FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_153.3520000005a498.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_153.3520000005a498.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132369
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA9250.698804100658009No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT5440.4109723575761696No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4270.32258308214158904No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCCTT4110.310495659859937No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3520.2659232901963451No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3380.2553467956998995No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC3230.24401483731085072No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2910.21983999274754665No Hit
CATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCT2860.21606267328453035No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2740.20699710657329135No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2670.20170885932506857No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2620.1979315398620523No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2470.1865995814730035No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA2460.18584411758040023No Hit
GTTCTGGATCAACCGTCCTTCATGATCACTGAGTTTAAGTTCAGTGCACA2400.1813113342247807No Hit
TAGTAATACTATGGTCTCTAATCGGCCAAAAATCACATTGAAATTCGCTT2350.17753401476176447No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2340.1767785508691612No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2330.17602308697655797No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2270.17149030362093842No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG2220.16771298415792218No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2120.16015834523188965No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2110.15940288133928637No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA2050.15487009798366688No Hit
AGCCAATAGAGTCCTACAGACTTTCCCAATGGAACCTTCTTCCACTCCTT2040.15411463409106363No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2010.15184824241325387No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2000.1510927785206506No Hit
ATCCAAAACAGCTTGACAATAGAGAAAATGGTACTCTCTGCTTTTGATGA1960.14807092295023758No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1930.14580453127242785No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1870.14127174791680833No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC1870.14127174791680833No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1820.13749442845379206No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC1820.13749442845379206No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1770.1337171089907758No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC1760.13296164509817254No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1760.13296164509817254No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1720.12993978952775953No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1660.12540700617214No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1640.1238960783869335No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1570.11860783113871072No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA1560.11785236724610748No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1550.11709690335350421No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG1470.11105319221267819No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAGAATAA1470.11105319221267819No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT1460.11029772832007495No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC1410.10652040885705868No Hit
TGTCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG1370.10349855328664567No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT1370.10349855328664567No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA1340.1012321616088359No Hit
GTTCAAGAGTGCTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT1330.10047669771623266No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCC100.0014747304245.00002245
CGGACGC100.0014747304245.00002245
GCAGGGG800.0153.1259
GCAGGGT1650.0141.060619
CGAAAGC1600.0122.500013
AGGGTGT602.0900188E-9122.499996
AGCAGGG3100.0114.596778
CAAAAGC7050.0111.205674
GCGAAAG1800.0108.888892
AAGCAGG8600.0108.2558067
TTCTGGA1050.0105.000012
TCGTACT354.4365416E-4105.02
AGCAAAA7350.0104.999992
GCAAAAG7850.098.31213
GTATTAA501.4894631E-597.999991
GTTCTGG1150.095.869571
AGCAGGT5450.094.403678
GTCGTAC407.5439335E-491.875011
AGCGAAA2150.091.162791
GAGCGAA1350.090.740741
Produced by FastQC (version 0.11.2)