FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_114.3520000005a455.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_114.3520000005a455.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144860
Sequences flagged as poor quality0
Sequence length251
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT10760.7427861383404667No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA6630.45768328040867046No Hit
GTATGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC6190.4273091260527406No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA4530.3127157255280961No Hit
TTTTTGGACAGTATGGATAACAAATAGTAGCACTGCTATAACTATTTTAA4480.3092641170785586No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC4120.2844125362418887No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT3840.2650835289244788No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA3430.23678033963827144No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3390.23401905287864147No Hit
CTTCCTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG3390.23401905287864147No Hit
ACTCTTCCTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATC2700.18638685627502416No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2660.1836255695153942No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2660.1836255695153942No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC2660.1836255695153942No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA2620.1808642827557642No Hit
ACTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGGGCCCTA2510.1732707441667817No Hit
ATATGCACTCACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCAT2460.16981913571724425No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA2410.16636752726770673No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2350.16222559712826176No Hit
ATATAAATCAGAAGCATGGCCCATTGGGGAGTCCCCCAAAGGAGTGGAAG2280.15739334529890928No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT2200.15187077177964933No Hit
GAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT2100.14496755488057436No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2080.14358691150075936No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2040.14082562474112936No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT2000.1380643379814994No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1940.1339224078420544No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT1890.1304707993925169No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1820.12563854756316445No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1820.12563854756316445No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1800.12425790418334944No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1770.12218693911362694No Hit
GGGTACACTATGGACACAGTCAACAGAACAAACACAAGCCAAAGGGGAAT1690.11666436559436698No Hit
GAACTCTTCCTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAA1690.11666436559436698No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA1680.11597404390445948No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA1640.11321275714482948No Hit
GGGTACACTATGGACACAGTCAACAGAACACACCAATACTCAGAGAGGGG1620.11183211376501451No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1610.111141792075107No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1600.1104514703851995No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1540.10630954024575452No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1510.10423857517603202No Hit
CTCTTCCTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCC1490.10285793179621705No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC1460.10078696672649455No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1450.10009664503658705No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAT100.0014748739245.00002245
GCCGACA100.0014748739245.00002245
GCAGGGG800.0168.43759
ACAGGTA150.0049625086163.333332
GCAGGGT1100.0155.909079
TAAATTC700.0140.09
CTAAATT750.0130.666678
GCAAAAG6950.0130.431663
AAGCAGG8350.0123.2335367
GTTAATG404.915477E-6122.500013
AGCAGGG2600.0117.788468
CAAAAGC7800.0116.217964
CAAGCTA851.8189894E-12115.294114
GTATTAA458.827848E-6108.888891
GCTAAAT901.8189894E-12108.888897
AAGCTAA901.8189894E-12108.888895
AGCAGGT5600.0102.81258
CGAAAGC1200.0102.0833364
GGTAGTT501.48994095E-598.07
ATTAATT552.3914394E-589.0909043
Produced by FastQC (version 0.11.2)