FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_107.3520000005a28d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_107.3520000005a28d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139437
Sequences flagged as poor quality0
Sequence length251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA10810.7752605119157756No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCGAATATC5380.38583733155475236No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4260.30551431829428344No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3880.27826186736662434No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3720.2667871511865574No Hit
GTACTGGAACTAGGATGAGTTCCAATGGCTCTCATTGCCTGCACCATCTG3690.2646356419027948No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG3690.2646356419027948No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTGTTTGGGTCTCCATTCCC3620.2596154535740155No Hit
GCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGC3480.2495750769164569No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3210.2302114933625939No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3020.2165852678987643No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG2930.21013074004747664No Hit
TTCCAGTACAGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2700.19363583553863034No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2620.18789847744859686No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2610.18718130768734267No Hit
CACTGGAGTTTAACAATGAAAGCTTCAATTGGGCTGGAGTCACTCAAAAC2590.1857469681648343No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC2560.18359545888107173No Hit
CTTCCTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG2550.18287828911981754No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2460.17642376126852988No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG2240.1606460265209378No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2230.15992885675968357No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2200.157777347475921No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT2180.15634300795341263No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCC2040.14630263129585405No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2020.14486829177334568No Hit
GGTTTGGCCTGCGGAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGG2000.1434339522508373No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1990.1427167824895831No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT1970.14128244296707473No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT1960.14056527320582055No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1930.13841376392205798No Hit
GGATTATGCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGT1910.13697942439954963No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC1910.13697942439954963No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1910.13697942439954963No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1840.1319592360707703No Hit
TTCCATGAGAGCCTCAAGATCTGTGTTTTTCCCAGCAAAGACATCTTCAA1720.12335319893572007No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG1670.11976735012944914No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG1660.11905018036819497No Hit
CCCTTCTACTTCTGAGAATTCTTTTTCAATCTGATGGAATTTCTCGTTGG1640.11761584084568659No Hit
CCCTTATTCTGGGTCTAGATCCGATATTCGGGATTACAGCTTGTTGGCTT1640.11761584084568659No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT1630.1168986710844324No Hit
CTCTTAGACCTGTACTGGAACTAGGATGAGTTCCAATGGCTCTCATTGCC1530.10972697347189055No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT1520.10900980371063634No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG1490.10685829442687378No Hit
TCCTAGTTCCAGTACAGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1450.10398961538185703No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAAAG5350.0141.962623
CGAAAGC1600.0137.81254
GCAGGTA4350.0129.540229
AAGCAGG7750.0126.4516147
GCAGGGT1100.0122.59
ACAAGGT704.3655746E-11122.58
AGCAGGT5150.0116.55348
CAAAAGC6750.0114.333334
GAGCGAA1950.0113.076931
GCGAAAG2100.0105.03
GTACTAA354.4370434E-4105.0245
AAGCTAA354.4370434E-4105.05
AGCAAAA7550.0102.2185442
AGCAGGG3150.097.222218
GCAGGGG1800.095.277779
CAAGCTA407.544784E-491.875014
GCTAAAT407.544784E-491.875017
GAGCAAA8500.090.794121
AGCGAAA2450.090.000012
AGGGTGT450.001204599281.6666646
Produced by FastQC (version 0.11.2)