FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_103.3520000005a206.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_103.3520000005a206.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123349
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA8470.6866695311676625No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT5740.46534629384915965No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3380.2740192462038606No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTGTTTGGGTCTCCATTCCC3030.24564447218866792No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2910.2359159782406019No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2620.21240545119944224No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG2530.20510908073839268No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA2460.19943412593535415No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2430.19700200244833765No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG2410.19538058679032666No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2400.19456987896132114No Hit
GTACTGGAACTAGGATGAGTTCCAATGGCTCTCATTGCCTGCACCATCTG2340.1897056319872881No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2220.17997713803922205No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2160.175112891065189No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2110.1710593519201615No Hit
TTCCAGTACAGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1930.15646661099806242No Hit
GTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAATGCATGTGTC1850.14998094836601836No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCGAATATC1790.14511670139198535No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC1780.14430599356297985No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1750.1418738700759633No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT1720.1394417465889468No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1700.1378203309309358No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1680.1361989152729248No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1650.13376679178590828No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1620.13133466829889176No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1560.12647042132485875No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1550.12565971349585323No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1540.12484900566684773No Hit
GTTAAAGCACAGATTTGAAATAATCGAAGGGAGAGACAGAACAATGGCCT1500.1216061743508257No Hit
GAATAACAAGGAGCAGTTTTCTTGACTCCGCTGAAAATCCTTCCAATTGT1500.1216061743508257No Hit
CTAAAAATCTACAGAGATTCGCTTGGAGAAGCAGTAATGAGAATGGGGGA1440.11674192737679268No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1430.11593121954778718No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1390.11268838823176516No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1380.11187768040275967No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT1380.11187768040275967No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1330.10782414125773213No Hit
CCCTAGAAGATCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA1330.10782414125773213No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGATACTGGGCCATAAGA1320.10701343342872661No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT1300.10539201777071561No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT1290.10458130994171011No Hit
GATCCTTAGAGACTCGAACTGTGTTATCATTCCATTCAAGTCCTCCGATG1250.1013384786256881No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAATT100.0014746084245.000028
AGGGTGT351.2007149E-8175.06
GCAGGGG700.0175.09
TCGTCGG301.1737066E-6163.33333245
CAAGCTA150.0049616187163.333334
CGAAAGC1300.0131.923084
GCAGGGT850.0129.705899
GCAAAAG6250.0125.443
GATCTAC501.0099393E-7122.56
CAAAAGC6800.0117.095594
AAGCAGG8400.0113.750017
AGCAGGG2200.0111.363648
GCAGGTA5050.0109.158429
AGCAGGT5900.0107.9660958
AGCAAAA7900.099.240512
GCGAAAG1950.087.948723
GAGCAAA9100.087.51
GAGCGAA2000.085.751
AAAGCAG11950.079.958156
ACAAGGG955.0818926E-877.3684163
Produced by FastQC (version 0.11.2)